May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
BDNF microsphere treatment increases functional effects of retinal transplants
B.B. Thomas; R.B. Aramant; G.T. Qiu; S. Arai; Z. Chen; S.R. Sadda; M.J. Mahoney; M.J. Seiler
Author Affiliations & Notes
  • B.B. Thomas
    Ophthalmology, Doheny Retina Institute, Keck Sch. Med.,USC, Los Angeles, CA
  • R.B. Aramant
    Anat. Sci. & Neurobiol., Univ. Louisville Sch. Med., Louisville, KY
  • G.T. Qiu
    Ophthalmology, Doheny Retina Institute, Keck Sch. Med.,USC, Los Angeles, CA
  • S. Arai
    Ophthalmology, Doheny Retina Institute, Keck Sch. Med.,USC, Los Angeles, CA
    Division of Ophthalmology and Vision Science, Graduated School of Medical and Dental Sciences, Niigata University, Niigata, Japan
  • Z. Chen
    Ophthalmology, Doheny Retina Institute, Keck Sch. Med.,USC, Los Angeles, CA
  • S.R. Sadda
    Ophthalmology, Doheny Retina Institute, Keck Sch. Med.,USC, Los Angeles, CA
  • M.J. Mahoney
    Chem. Engineering, Univ. Colorado, Boulder, CO
  • M.J. Seiler
    Ophthalmology, Doheny Retina Institute, Keck Sch. Med.,USC, Los Angeles, CA
    Cell & Neurobiol., Keck Sch. Med., USC, Los Angeles, CA
  • Footnotes
    Commercial Relationships  B.B. Thomas, None; R.B. Aramant, Ocular transplantation LLC P; G.T. Qiu, None; S. Arai, None; Z. Chen, None; S.R. Sadda, None; M.J. Mahoney, None; M.J. Seiler, Ocular transplantation LLC P.
  • Footnotes
    Support  03040
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 5184. doi:
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      B.B. Thomas, R.B. Aramant, G.T. Qiu, S. Arai, Z. Chen, S.R. Sadda, M.J. Mahoney, M.J. Seiler; BDNF microsphere treatment increases functional effects of retinal transplants . Invest. Ophthalmol. Vis. Sci. 2004;45(13):5184.

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Abstract

Abstract: : Purpose: To test whether coating with brain derived neurotrophic factor (BDNF) microspheres could improve the functional effect of fetal retinal sheet transplantation in transgenic (rhodopsin mutant) S334ter–3 rats. Methods: S334ter–3 rats were exposed to blue light for 5 days to accelerate photoreceptor degeneration. At postnatal day (P) 29–43, the rats received in one eye subretinal transplants of retinal sheets from embryonic day 18–19 pigmented normal rats coated with BDNF microspheres. Beginning two weeks after surgery, visual acuity was monitored using an optokinetic head tracking apparatus. In addition, visual responses were recorded electrophysiologically from the superior colliculus (SC) at P78–98. Controls were rats without surgery or receiving BDNF microspheres only. Results: Rats that received BDNF coated transplants showed significant improvement in the optokinetic test (P<0.05, transplanted vs non–transplanted eye). With low light (1cd/m2) to preferentially stimulate rods, responses were recorded from the SC in a restricted area corresponding to the retinal location of the transplant. With stronger light (2500 cd/m2), responses were recorded from a larger SC area. The onset latency and the amplitude of the responses to low and strong light were close to that of the normal rats. Controls receiving only BDNF without transplants showed improvement in the optokinetic response in some of the rats; however, no responses from the SC with low light. No–surgery rats had no improvements in either test. Conclusions: BDNF treatment resulted in better optokinetic and visual electrophysiological responses to bright light (photopic), presumably due to rescue of host cones. In the transplant groups, both BDNF treated and non–treated rats showed visual responses to low light, possibly due to a direct effect of the transplant rods. Supported by: Foundation Fighting Blindness, Anonymous Sponsor, Foundation for Retinal Research, Fletcher Jones Foundation, NIH EY03040.

Keywords: transplantation • retina • superior colliculus/optic tectum 
© 2004, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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