May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Iris–derived cells from adult rodents and primates adopt photoreceptor–specific phenotypes
Author Affiliations & Notes
  • T. Akagi
    Ophthalmology & Visual Sciences,
    Institute for Virus Research,
    Kyoto University, Kyoto, Japan
  • J. Akita
    Ophthalmology & Visual Sciences,
    Kyoto University, Kyoto, Japan
  • M. Haruta
    Ophthalmology & Visual Sciences,
    Kyoto University, Kyoto, Japan
  • Y. Honda
    Ophthalmology & Visual Sciences,
    Kyoto University, Kyoto, Japan
  • R. Kageyama
    Institute for Virus Research,
    Kyoto University, Kyoto, Japan
  • M. Yamada
    Tokyo Metropolitan Institute of Technology, Hino, Japan
  • M. Takahashi
    Ophthalmology & Visual Sciences,
    Translational Research Center,
    Kyoto University, Kyoto, Japan
  • Footnotes
    Commercial Relationships  T. Akagi, None; J. Akita, None; M. Haruta, None; Y. Honda, None; R. Kageyama, None; M. Yamada, None; M. Takahashi, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 5298. doi:
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      T. Akagi, J. Akita, M. Haruta, Y. Honda, R. Kageyama, M. Yamada, M. Takahashi; Iris–derived cells from adult rodents and primates adopt photoreceptor–specific phenotypes . Invest. Ophthalmol. Vis. Sci. 2004;45(13):5298.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Iris tissue in the adult rat eye has the potential to express a photoreceptor–like phenotype following Crx induction. To explore potentials of iris cells as a source for retinal transplantation, we investigated the effects of various genes that have been reported to affect photoreceptor development. Methods: We prepared iris–derived cell culture from adult DA rat and cynomolgus monkey iris tissues. We first performed immunohistochemistry to see whether native iris–derived cells possessed some characteristics of retinal progenitor cells. We next used replication–incompetent retroviruses to examine the effects following the expression of Crx, NeuroD or Nrl alone, or the combination of Crx and NeuroD or Crx and Nrl, by performing immunocytochemistry, RT–PCR and intracellular recording analyses. To further examine whether iris–derived cells could integrate into host retina to exhibit a photoreceptor–specific phenotype after in vitro gene transfer, we transplanted the transfected rat–iris–derived cells into embryonic explanted retinas. Results: Adult iris–derived cells of both rodents and primates showed retinal progenitor properties. Expression of Crx alone induced photoreceptor–specific genes in adult rat iris cells. For monkey–iris–derived cells, both of Crx and NeuroD were essential to adopt a photoreceptor–specific phenotype. The photoreceptor–like cells derived from iris tissue after genes transfer produced rod photoreceptor–specific electrophysiological response to light stimuli in vitro. We further found that iris–derived cells were able to integrate into the developing host retina after gene transfer. Conclusions:Iris cells, which can be easily obtained, are good candidate as a source for autologous retinal transplantation in degenerative retinal diseases.

Keywords: photoreceptors • iris • transcription factors 
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