Abstract
Abstract: :
Purpose: Normal development of the mammalian retina depends on the proliferation of retinal progenitor cells. These cells must undergo extensive cell division in a process that is dependent on the Chx10 gene. Although Chx10 is known to genetically interact with the cell cycle, its precise function is still not known. The goal of this study is to determine at what level of proliferation control the Chx10 protein acts. Methods: To do this, we have examined the cell cycle parameters of Chx10 null retinal progenitors, and the proliferative responses of these cells to known mitogens. Results: We find that Chx10 null progenitors have abnormal cell cycle kinetics in specific phases. Additionally, we find that in explant cultures Chx10 null progenitors can respond to several mitogens that use different signal transduction pathways. Conclusions: These results indicate that Chx10 is necessary for normal cell cycle progression, but that it is not essential for proliferative responses to many mitogens.
Keywords: retinal development • retinal culture • cell–cell communication