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J. Ge, K. Yu, J. Zhuang, Z. Wang, L. Yu; The Inducing Action of Intraocular Microenviroment . Invest. Ophthalmol. Vis. Sci. 2003;44(13):149.
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© ARVO (1962-2015); The Authors (2016-present)
Objective To investigate the induction action of intraocular microenviroment of vitreous cavity and subretinal space on the adult rat bone marrow stromal cells(BMSCs ) for further investigation of an alternative way for the treatment of glaucomatous optic atrophy and other neuroretinopathy. MethodsThe full marrow from adult Wistar rat were cultured and passaged repeatedly to harvest the pure BMSCs.In vivo, the BMSCs labeled with BrdU were injected into vitreous cavity and subretinal space.,the control grup with injection of DMEM media. The eye balls were enucleated after 1 and 4weeks for histopathological and immunocytochemical examination to identify the charactaritics of the induced cells. ResultsThe BMSCs injected into intravitreous and subretinal space can be induced into the retinal neuron -like cells and without inflammatory reaction, immune-mediated rejection and toratomas formation. ConclusionsThe BMSCs from adult rat can be induced into retinal cells in the intraocular microenvironment. It suggests that the BMSCs would be an alternative seeding cells of the cell therapy to treat the glaucomatous degeneration and other neuroretinopathy.
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