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C. Gonzalez, V. Fernandez, J. Parel; Measurement of Human, Primate and Rabbit Lens Capsule Thickness using Histology . Invest. Ophthalmol. Vis. Sci. 2003;44(13):249.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To compare the lens capsule thickness of several animal models for lens refilling surgery. Methods: 15 lenses were explanted from cadaver eyes (2 cynomolgous monkey, 4 human and 9 rabbit), placed in Formalyn and processed for histology. Alcohol cycling was used to dehydrate the tissue prior to embedding the lenses in paraffin. The encased lenses were then microtomed and the sections placed on slides for microscope analysis. The slides were stained using periodic acid-schiff (PAS) and Hematoxilin and Eosin (H&E;) to enhance the lens capsule appearance. Digital images of the lenses' anterior, peripheral, and posterior capsule chambers were taken at 40X magnification and scaled to a ruler in the image. The photographs were measured using a computer graphics program with a measurement resolution of ±0.1µm. Results: Human, rabbit, and cynomolgus capsule thicknesses were 11.9±3.3 µm, 12±6.0 µm, 7.7±2.1 µm for the central anterior capsule; 5.7±4.0 µm, 6.2±2.4 µm, 3.8+/-2.7 µm for the central posterior capsule; 6.1±1.3 µm, 5.5±1.4 µm, 4.0+/-1.3 µm for the equatorial capsule. Conclusions:Human and rabbit capsule had comparable thickness. Cynomolgus monkey capsules were 35% thinner than rabbit and human lenses in all locations. Support: NIH Grant#EY14225; Australian CRC Scheme, CRC for Eye Research and Technology, Sydney, Australia; Florida Lions Eye Bank; Henri and Flore Lesieur Foundation; Research to Prevent Blindness.
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