May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Two Techniques to Evaluate the Efficiency of Various Substances in the Prevention of Posterior Capsular Opacification (PCO)
M.A. Orozco; V. Fernandez; M. Fragoso; P. Lamar; E. Hernandez; P. Milne; J.M. Parel; M. Willcox
Author Affiliations & Notes
  • M.A. Orozco
    Bascom Palmer Eye Institute and Department of Biomedical Engineering, University of Miami, Miami, FL, United States
  • V. Fernandez
    Ophthalmic Biophysics Center and Department of Ophthalmology, Bascom Palmer Eye Institute, Miami, FL, United States
  • M. Fragoso
    Ophthalmic Biophysics Center and Department of Ophthalmology, Bascom Palmer Eye Institute, Miami, FL, United States
  • P. Lamar
    Ophthalmic Biophysics Center and Department of Ophthalmology, Bascom Palmer Eye Institute, Miami, FL, United States
  • E. Hernandez
    Ophthalmic Biophysics Center and Department of Ophthalmology, Bascom Palmer Eye Institute, Miami, FL, United States
  • P. Milne
    Ophthalmic Biophysics Center and Department of Ophthalmology, Bascom Palmer Eye Institute, Miami, FL, United States
  • J.M. Parel
    Ophthalmic Biophysics Center and Department of Ophthalmology, Bascom Palmer Eye Institute, Miami, FL, United States
  • M. Willcox
    Opthalmology, Australian Cooperative Research Centre (CRC) Scheme, CRC for Eye Research and Technology, Sydney, Australia
  • Footnotes
    Commercial Relationships  M.A. Orozco, None; V. Fernandez, None; M. Fragoso, None; P. Lamar, None; E. Hernandez, None; P. Milne, None; J.M. Parel, None; M. Willcox, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 280. doi:
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      M.A. Orozco, V. Fernandez, M. Fragoso, P. Lamar, E. Hernandez, P. Milne, J.M. Parel, M. Willcox; Two Techniques to Evaluate the Efficiency of Various Substances in the Prevention of Posterior Capsular Opacification (PCO) . Invest. Ophthalmol. Vis. Sci. 2003;44(13):280.

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Abstract

Abstract: : Purpose: To assess efficiency of two different techniques and various substances in the prevention of PCO. Methods: 33 NZW rabbits (2.31±0.91 kg.) were used. Lens material was removed through a micro-capsulorhexis and the empty capsular bag was refilled (Phaco-Ersatz surgey). In group 1 of animals (10 rabbits) the micro-capsulorhexis was left open. In group 2, a Mini Capsulorhexis Valve (MCV) was placed to seal the capsulorhexis. In both groups a mixture of viscoelastic (1.4% sodium hyaluronate, SHA, Corneal SA, France) and substance was injected into the empty lens capsule before refilling. The mixture was removed after 3 min, and the empty bag was rinsed with BSS and refilled with pure SHA. Double-distilled water was used as a substance for Group 1. The following substances were used for group 2: SHA only (control, n=1), Mitomycin-C (0.2mg/ml, n=7), EDTA (10mM and 15mM, n=5), 5-flurouracil (5-FU, 33mg/ml, n=2), 0.003% acetic acid (n=8), and double distilled water (n=10). Postoperative follow-up to evaluate PCO was done at 1, 7, 14, 21, 28 and 35 days, when animals were euthanized. Results: PCO and lens regrowth were observed in both groups. Group 1 presented PCO earlier than Group 2. In Group 1, PCO appeared between day 7 and 36. For Group 2 the order of appearance of PCO was: EDTA 15mM (POD1), SHA (POD 7), Mitomycin-C (POD 15), acetic acid (0.3%) (POD 15), acetic acid (3%) (POD 15), saline (POD 15), acetic acid 0.003% (POD 22), 5-FU (POD 22), EDTA10mM (POD22) and double distilled water (POD29). Conclusion:MCV is effective in delaying the appearance of PCO in the rabbit. EDTA and distilled water presented the longest delay of PCO formation when the lens was fitted with a MCV. CR: None: Support: Henri and Flore Lesieur Foundation; the Florida Lions Eye Bank; Research to Prevent Blindness; Australian Cooperative Research Centre (CRC) Scheme, CRC for Eye Research and Technology, Sydney.

Keywords: posterior capsular opacification (PCO) • proliferation • cell death/apoptosis 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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