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M.M. Fung, K. Bluethner, U. Mayer; The Influence of Hypericin and UVA-Light on Bovine Lensepithelium In Vitro With Interaction of Catalase . Invest. Ophthalmol. Vis. Sci. 2003;44(13):301.
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Purpose: To evaluate the effect of UVA-Light activated Hypericin on bovine lens epithelium and the cell protective effect of Catalase. Background: Hypericin, a polycyclic aromatic naphthodianthron, produces singlet oxygen and other excited state intermediates. Hypericin has shown to have antiretroviral, antibacterial and antitumoral effects in vitro photoactivated by UVA-light and visible light. There is a possibility of using hypericin as a photodynamic agent for the treatment of eye tumors and eye viral and bacterial diseases. Hypericin induces photosensitised erythema in the skin. Hypericin absorbs light in the range of visible and UVA-light, so it can damage retina and lens. Hypericin is a compound of antidepressive drugs. Material and Methods: 246 cultures of bovine lens epithelium, Medium TCM 199 plus 20% fetal calf serum, 2% glutamine, 5% Hepes buffer, 1% Penicillin-Streptomycin, Amphotericin B. Bovine lens epithelium was irradiated in the presence of hypericin (1 x 10-5 M) and in the presence and absence of UVA- light (300 - 400 nm, 740.7 J/cm2). 48 h after irradiation the surviving cells were dyed by trypan blue and counted in Neubauer's chamber. The results were statistically evaluated by Mann-Whitney-U-Test. Results: a) Hypericin inhibits bovine lens epithelium cell in darkness significantly with a dosage > 1 x 10-5 M b) 1 x 10-5 M Hypericin, photoactivated by UVA-Light, induces irreversible phototoxic damage to bovine lens epithelium in vitro. This cell damage is significantly stronger than cell damage induced by UVA-light alone. c) The addition of catalase had a significant inhibitory effect on the photodynamic cytotoxicity of Hypericin. Conclusions: Photoactivated Hypericin induces irreversible phototoxic damages to bovine lens epithelium in vitro. Added catalase protects the cells significantly from them. Further studies in animal models regarding lens damage are advisable.
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