May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Time Dependency of In Vivo Ultraviolet Radiation (UVR)-Induced Lens Metabolic Changes in the Rat as Analyzed by NMR Spectroscopy
Author Affiliations & Notes
  • S. Löfgren
    St Eriks Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • M.K. Kakar
    St Eriks Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • Ø. Risa
    Faculty of Natural Science and Technology, University of Science and Technology, Trondheim, Norway
  • A. Midelfart
    Faculty of Medicine, University of Science and Technology, Trondheim, Norway
  • O. Sæther
    Faculty of Medicine, University of Science and Technology, Trondheim, Norway
  • J. Krane
    Faculty of Medicine, University of Science and Technology, Trondheim, Norway
  • V.C. Mody Jr
    Faculty of Medicine, University of Science and Technology, Trondheim, Norway
  • P.G. Söderberg
    Faculty of Medicine, University of Science and Technology, Trondheim, Norway
  • Footnotes
    Commercial Relationships  S. Löfgren, None; M.K. Kakar, None; Ø. Risa, None; A. Midelfart, None; O. Sæther, None; J. Krane, None; V.C. Mody Jr, None; P.G. Söderberg, None.
  • Footnotes
    Support  Health and Rehabilitation Grant, Norway
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 303. doi:
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      S. Löfgren, M.K. Kakar, Ø. Risa, A. Midelfart, O. Sæther, J. Krane, V.C. Mody Jr, P.G. Söderberg; Time Dependency of In Vivo Ultraviolet Radiation (UVR)-Induced Lens Metabolic Changes in the Rat as Analyzed by NMR Spectroscopy . Invest. Ophthalmol. Vis. Sci. 2003;44(13):303.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To investigate time dependency of metabolic changes in rat lenses exposed to ultraviolet radiation-B (UVR-B), using High Resolution Magic Angle Spinning (HR MAS) NMR spectroscopy. Methods: Eighty pigmented rats were divided into four groups and unilaterally exposed to 15 kJ/m2 UVR-B for a duration of 15 minutes. After 5, 25, 125, and 625 h, respectively, the lenses were isolated and the degree of cataract was quantified by measuring the amount of forward light scattering. The lenses (control and exposed) from five rats of each group were analyzed for a range of metabolites by HR MAS NMR spectroscopy, using the lenses in situ. Results: The UVR-exposed lenses in all groups developed statistically significant light scattering, peaking at 25 h and decreasing to near-control values at 625 h. The whole lens concentrations of several metabolites peaked at 125 hours, with valine, phenylalanine, and tyrosine increasing significantly. Taurine, betaine, myoinositol, choline, and succinate, however, decreased significantly at 125 h. All investigated metabolites exhibited a decrease at 625 h. Lens concentrations of glutathione was lowered at all time points, although not significant. Lactate concentrations remained unchanged. Conclusions: Cataract induced by a moderate UVR-B dose in pigmented rats is transient, peaking earlier than shown for albino rats. The major change in metabolic profile occurs at 125 and 625 h, with a general trend of decrease in metabolite concentration at 625 h. HR MAS NMR spectroscopy is a potentially effective tool for analyzing a wide spectrum of metabolites in whole lens tissue.

Keywords: cataract • radiation damage: light/UV • animal model 
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