May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
-Melanocyte-stimulating Hormone (MSH) as an Inhibitor of Traumatic Ocular Inflammation in Rats
D. Wasilewski; J. Cui; R.E. Smith; L. Rife; M. Mahmoud; S.C. Yiu
Author Affiliations & Notes
  • D. Wasilewski
    Ophthalmology, Ocular Surface Center, Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States
  • J. Cui
    Ophthalmology, Ocular Surface Center, Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States
  • R.E. Smith
    Ophthalmology, Ocular Surface Center, Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States
  • L. Rife
    Ophthalmology, Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States
  • M. Mahmoud
    Ophthalmology, Doheny Retina Institute, Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States
  • S.C. Yiu
    Ophthalmology, Doheny Retina Institute, Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States
  • Footnotes
    Commercial Relationships  D. Wasilewski, None; J. Cui, None; R.E. Smith, None; L. Rife, None; M. Mahmoud, None; S.C. Yiu, None.
  • Footnotes
    Support  Baxter Junior Faculty Award, Research to Prevent Blindness, and Symbigene, Inc.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 343. doi:
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      D. Wasilewski, J. Cui, R.E. Smith, L. Rife, M. Mahmoud, S.C. Yiu; -Melanocyte-stimulating Hormone (MSH) as an Inhibitor of Traumatic Ocular Inflammation in Rats . Invest. Ophthalmol. Vis. Sci. 2003;44(13):343.

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Abstract

Abstract: : Purpose: α-Melanocyte-stimulating hormone (α-MSH) is an endogenous neuroimmunomodulatory peptide that inhibits all major forms of experimental inflammation. We studied whether it can reduce the inflammatory reaction after induced ocular trauma in rats. Methods: Fifty-seven male Sprague Dawley rats, weighing 120g to 196g, were divided into 6 groups. All rats received a non penetrating 2 mm-long corneo-limbal incision, followed by paracentesis and aspiration of aqueous humor. Topical α-MSH (10 µL) was applied every 8 hours. Five concentrations were used (10-8M, 10-7M, 10-6M, 10-5M and 10-4M ), with each of five groups receiving a different concentration and the control group receiving balanced salt solution. Twenty-four hours after injury, we measured the protein level using the Bio-Rad reagent, counted total inflammatory cells using a hemacytometer, and measured clinical inflammation, on a scale of 1 to 4, by observing conjunctival hyperemia and edema through the operating microscope. Additional evaluation was done morphometrically using a digital camera. The aspirated aqueous humor was kept on ice and samples analyzed immediately after the study. Results: Topical α-MSH at all five concentrations reduced clinical inflammation, protein levels and total inflammatory cells in the aqueous humor. α-MSH reduced the concentration of protein and inflammatory cells in the anterior chamber in a dose-response manner. The EC50 of MSH was 12.4 nM and 17.7 nM for the inhibition of protein and inflammatory cells, respectively. The mean reduction of clinical inflammation was 75.5% in the treatment groups, versus 59.3% in the control group ( P<0.05 ). Conclusions: Topical α-MSH reduced the total number of inflammatory cells and the protein levels in aqueous humor, and it reduced the clinical signs of acute ocular inflammation. The results suggest that topical α-MSH might be a useful agent against acute ocular inflammatory reaction.

Keywords: inflammation • anterior segment • wound healing 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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