May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Upregulation of Fibronectin EDA in RPE Cells by Transforming Growth Factor Beta (TGF-b)
R.R. Khankan; C. Spee; S. He; S.J. Ryan; D.R. Hinton
Author Affiliations & Notes
  • R.R. Khankan
    Pathology, Doheny Eye Institute Keck Sch Med U Southern Calif, Los Angeles, CA, United States
  • C. Spee
    Ophthalmology, Doheny Eye Institute Keck Sch Med U Southern Calif, Los Angeles, CA, United States
  • S. He
    Ophthalmology, Doheny Eye Institute Keck Sch Med U Southern Calif, Los Angeles, CA, United States
  • S.J. Ryan
    Ophthalmology, Doheny Eye Institute Keck Sch Med U Southern Calif, Los Angeles, CA, United States
  • D.R. Hinton
    Ophthalmology, Doheny Eye Institute Keck Sch Med U Southern Calif, Los Angeles, CA, United States
  • Footnotes
    Commercial Relationships  R.R. Khankan, None; C. Spee, None; S. He, None; S.J. Ryan, None; D.R. Hinton, None.
  • Footnotes
    Support  NIH grants EY03040 & EY01545; Research to Prevent Blindness; the Arnold & Mabel Beckman Foundation.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 376. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Upregulation of Fibronectin EDA in RPE Cells by Transforming Growth Factor Beta (TGF-b)
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      R.R. Khankan, C. Spee, S. He, S.J. Ryan, D.R. Hinton; Upregulation of Fibronectin EDA in RPE Cells by Transforming Growth Factor Beta (TGF-b) . Invest. Ophthalmol. Vis. Sci. 2003;44(13):376.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose: One of the earliest extracellular matrix (ECM) components expressed by RPE cells in response to injury is an alternatively spliced embryonic isoform of fibronectin, FN EDA. The purpose of this study was to determine the time and dose response effect of TGF-ß on FN EDA expression. Methods: Human fetal RPE cell cultures (passages 2-4) were exposed to increasing concentrations of both TGF-ß1 and TGF-ß2. FN EDA expression was analyzed at different time points in both cell supernatant and homogenates. Western blot and immunohistochemical analyses were performed utilizing a FN EDA-specific monoclonal antibody. Results: TGF-ß stimulation of RPE cells resulted in a marked dose-dependent increase in FN EDA that increased further with longer exposure to TGF-ß. Results were similar for both TGF-ßs. Conclusions: TGF-ß stimulates expression of FN EDA in RPE. Since TGF-ß is increased in a variety of ocular disorders, this result provides a mechanism by which TGF-ß may contribute to disease pathogenesis with an accumulation of ECM.

Keywords: retinal pigment epithelium • growth factors/growth factor receptors • eye movements 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept