May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Up-Regulation of Cyclooxygenase-2 in N-Methyl-D-Aspartate-Treated Rat Retina
Author Affiliations & Notes
  • A.E. Rosenfeld
    Ophthalmology, Univ. of Rochester School of Medicine, NY, USC/Doheny Eye Institute, Los Angeles, CA, United States
  • R.N. Sanchez
    Ophthalmology, USC/Doheny Eye Institute, Los Angeles, CA, United States
  • J. Wang
    Ophthalmology, USC/Doheny Eye Institute, Los Angeles, CA, United States
  • J.M. Kwong
    Ophthalmology, UCLA/Jules Stein Eye Institute, Los Angeles, CA, United States
  • A.A. Sadun
    Ophthalmology, UCLA/Jules Stein Eye Institute, Los Angeles, CA, United States
  • T.T. Lam
    Ophthalmology, UCLA/Jules Stein Eye Institute, Los Angeles, CA, United States
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 469. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      A.E. Rosenfeld, R.N. Sanchez, J. Wang, J.M. Kwong, A.A. Sadun, T.T. Lam; Up-Regulation of Cyclooxygenase-2 in N-Methyl-D-Aspartate-Treated Rat Retina . Invest. Ophthalmol. Vis. Sci. 2003;44(13):469.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: We examined the expression of cyclooxygenase-2 (COX-2) in rat retinas infused with the glutamatergic agonist N-methyl-D-aspartate (NMDA). Methods: Intravitreal injections of 2 µL of either 40 mM neutralized NMDA in 0.1M phosphate buffered saline or the vehicle alone were given to Sprague Dawley rats, 45-55 days old. Groups of animals were euthanized at 0, 3, 6, 9, 12, 18, 20, 24 and 36 hours and their eyes were enucleated. The retinas of some eyes were fixed, processed, embedded in paraffin, and sectioned. Immunohistochemistry for COX-2 was performed on these sections. Retinas of the remaining eyes were dissected and processed for Real-time RT-PCR analysis of COX-2. Results: Eyes injected with NMDA showed many COX-2-positive cells in the retinal ganglion cell layer and in the inner plexiform layer 18 hours after the infusion, while only a few COX-2-positive cells were noted in the control normal retinas. Semiquantitative Real-time RT-PCR demonstrated an up-regulation, above that of normal retinas, of COX-2 mRNA 6 to 12 hours after treatment with NMDA. Conclusions: COX-2 is up-regulated in the rat retina after NMDA-induced excitotoxicity.

Keywords: cytokines/chemokines • retinal degenerations: cell biology • immunohistochemistry 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×