May 2003
Volume 44, Issue 13
ARVO Annual Meeting Abstract  |   May 2003
Plasmin Produces Proangiogenic Fragments
Author Affiliations & Notes
  • E. Van Aken
    Ophthalmology, Ghent University, Gent, Belgium
  • L. Morbidelli
    Pharmacology, Institute of Pharmacological Sciences, Sienna, Italy
  • L. De Rijcke
    Laboratory of Experimental Cancerology, Ghent University, Gent, Belgium
  • M. Ziche
    Laboratory of Experimental Cancerology, Ghent University, Gent, Belgium
  • M. Mareel
    Laboratory of Experimental Cancerology, Ghent University, Gent, Belgium
  • J. De Laey
    Laboratory of Experimental Cancerology, Ghent University, Gent, Belgium
  • Footnotes
    Commercial Relationships  E. Van Aken, None; L. Morbidelli, None; L. De Rijcke, None; M. Ziche, None; M. Mareel, None; J. De Laey, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 556. doi:
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    • Get Citation

      E. Van Aken, L. Morbidelli, L. De Rijcke, M. Ziche, M. Mareel, J. De Laey; Plasmin Produces Proangiogenic Fragments . Invest. Ophthalmol. Vis. Sci. 2003;44(13):556.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: Angiogenesis produces new vessels from endothelial cells that invade from pre-existing vessels. Endothelial cells express two types of Ca2+ dependent cell-cell adhesion molecules: V(ascular)E(ndothelial)-cadherin, specific for endothelial cells, and N(eural)-cadherin, present also in retinal cells, fibroblasts, myocytes, pericytes. Ectodomain shedding releases soluble fragments (sN-CAD) from the extra-cellular domain of N-cadherin. We investigated whether sN-CAD would stimulate the invasion of endothelial cells and so exert a pro-angiogenic activity. Methods: ARM cells are mouse sarcoma cells transfected with cDNA encoding chicken N-cadherin; when treated with plasmin their conditioned medium is a soure of sN-CAD, that can be removed by immunodepletion. The rabbit cornea assay and chorio-allantoic membrane assay are used as in vivo angiogenesis assays. Results: ARM-conditioned medium stimulates angiogenesis in the chick chorio-allantoic membrane and the rabbit cornea, and this pro-angiogenic activity is abolished by immunodepletion of the conditioned medium. 10-mer HAV-comprising peptide, identical to aminoacid 235 to 244 from human N-cadherin, revealed the HAV sequence as crucial for the pro-angiogenic effect of sN-CAD. In the rabbit cornea, high doses (10µg and 50µg) of the HAV-comprising peptide, stimulated angiogensis, and a low dose (200ng) potentiated FGF-2 stimulated angiogenesis. Conclusions: Our results suggest that ectodomaine shedding of N-cadherin stimulates angiogenesis.

Keywords: neovascularization • proteolysis • retina 

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