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S. Ooto, M. Haruta, Y. Honda, H. Kawasaki, Y. Sasai, M. Takahashi; Induction of the Differentiation of Lentoids from Primate Embryonic Stem Cells . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1229.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To produce lens cells from primate embryonic stem (ES) cells in a reproducible controlled manner. Methods: Cynomologus monkey ES cells were induced to differentiate by stromal cell-derived inducing activity (SDIA). The lentoids produced by this treatment were processed for immunohistochemical and immunoblotting analysis. We also examined the effect of varying the concentration of fibroblast growth factor 2 (FGF-2) and the density of the ES colonies plated on the differentiation process. Results: Following a 2-3 week induction period, lentoids were produced by a subpopulation of ES colonies. Western blotting and immunohistochemistry revealed that these lentoids expressed crystallin alpha. The number of lentoids resulting from treatment increased with increasing FGF-2 concentration and plated colony density. Conclusions: The differentiation of primate ES cells into lentoids can be achieved by treatment with stromal cell-derived inducing activity. ES cells can be used to facilitate a greater understanding of the mechanisms functioning in differentiation in vivo and in vitro.
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