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J.D. Clough, C.H. Parikh, H.F. Edelhauser; Anterior Chamber, Lens and Globe Volumes in Balb/C and C57/BL6 Mice . Invest. Ophthalmol. Vis. Sci. 2003;44(13):648.
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Purpose: To quantify and compare the volumes of the anterior chamber (AC), lens and whole globe of Balb/C and C57/BL6 mice. Current ophthalmologic research requires injection of solutions containing antibodies, cells, or other biological materials into the AC of the mouse eye. However, the volume that the AC can actually hold has not been previously reported. It is possible, therefore, that researchers are unknowingly injecting volumes in excess of chamber size. Methods: We measured the volumes of the AC and the globe in 48 C57/B6 (age 9-17 weeks) and 11 Balb/C (age 9-20 weeks) mouse eyes. The eyes were enucleated post-mortem and photographed. The photographs were scanned to create digital images of the eyes. Lenses were dissected from the globe (12 C57/B6 and 14 Balb/C) and each lens was mounted on a slide to allow measurement of all diameters. Each lens was photographed and scanned. Using Scion Image, volumes of the AC, lens and globe were determined by measuring the number of pixels for the necessary parameters. Results: The AC was found to be significantly greater (P = 0.02) in Balb/C mice (µ = 3.08 + 0.78 µl) versus C57/B6 mice (µ = 2.39 + 0.73 µl). Volumes of the globe were not significantly different (P = .69) in C57/B6 (µ = 24.76 + 3.15 µl) and Balb/C (µ = 24.33 + 3.43 µl). Lens volumes were also not significantly different (P = .53) in C57/B6 (µ = 5.46 + 1.29 µl) and Balb/C (µ = 5.76 + 1.02 µl). Based on these volumes we estimate the volume of the vitreous to be between 10-12 µl. Conclusions: These findings give an approximation of the maximum volume of fluid that may be administered intracamerally and intravitreally in either Balb/C or C57/B6 mice. Researchers injecting solutions in excess of these volumes should not assume that the entire solution can be accommodated by these chambers.
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