May 2003
Volume 44, Issue 13
ARVO Annual Meeting Abstract  |   May 2003
The Effect of Lithium During Zebrafish Eye Development
Author Affiliations & Notes
  • H. Choi
    Lab. of Ophthalmology & Visual Sciense, Catholic Medical Univ, Seoul, Republic of Korea
  • C. Joo
    Lab. of Ophthalmology & Visual Sciense, Catholic Medical Univ, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  H. Choi, None; C. Joo, None.
  • Footnotes
    Support  Korean Ministry of Science and Technology grant (NRPD 2000-N-NL-01-C-121)
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 651. doi:
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      H. Choi, C. Joo; The Effect of Lithium During Zebrafish Eye Development . Invest. Ophthalmol. Vis. Sci. 2003;44(13):651.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: The Wnt/beta-catenin signaling plays multiple roles during embryonic development. Gsk3-beta(glycogen synthase kinase3-beta) plays a key role in cell-fate decisions by negatively regulating beta-catenin. It has been reported that lithium inhibit the Gsk3-beta activity thus mimicking Wnt signaling in zebrafish embryos. The purpose of this study is to search of differentially regulated gene(s) by lithium during zebrafish eye development. Methods: Treatment of zebrafish embryos with lithium after 4~6 hours post-fertilization leads to eyeless or small eyes. Total RNA was isolated from lithium treated embryos and untreated as a control. These RNA were used for cDNA synthesis followed by subtraction. Results: Approximately 700 clones from the cDNA subtraction libraries were blotted onto nitrocellulose membrane and hybridized with DIG-labeled cDNA from each group as probes. More than putative 70 individual clone were isolated and then sequenced. Among them the transcriptional levels of Type I cytokeratin, Type II cytokeratin and Ke3 were decreased in lithium treated embryos compared to untreated control group as determined by Northern analysis. This result is consistent with previous report that Type I cytokeratin and Type II cytokeratin were expressed in zebrafish eye. Conclusions: Our finding suggests that lithium may affect the expression of gene(s) responsible for eye formation during zebrafish early development.

Keywords: gene/expression • molecular biology • signal transduction 

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