May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Screening Chemokine Receptors on a Conjunctival Cell Line by Intracellular Calcium Measurement
Author Affiliations & Notes
  • M. Di Nolfo
    Dept Ophthalmology III, Quinze-Vingts National Hospital, Paris VI University, Paris, France
  • A. Lombet
    INSERM Unit 339, Saint Antoine Hospital, Paris VI University, Paris, France
  • C. Blondin
    INSERM Unit 339, Saint Antoine Hospital, Paris VI University, Paris, France
  • W. Rostène
    INSERM Unit 339, Saint Antoine Hospital, Paris VI University, Paris, France
  • C. Baudouin
    INSERM Unit 339, Saint Antoine Hospital, Paris VI University, Paris, France
  • F. Brignole
    Dept Toxicology, Faculty of Pharmaceutical Sciences, Paris V University, Paris, France
  • Footnotes
    Commercial Relationships  M. Di Nolfo, None; A. Lombet, None; C. Blondin, None; W. Rostène, None; C. Baudouin, None; F. Brignole, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 704. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M. Di Nolfo, A. Lombet, C. Blondin, W. Rostène, C. Baudouin, F. Brignole; Screening Chemokine Receptors on a Conjunctival Cell Line by Intracellular Calcium Measurement . Invest. Ophthalmol. Vis. Sci. 2003;44(13):704.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose : Chemokines are peptides with chemotactic properties which confer them an important role in the inflammatory reaction. We have sought for a wide variety of chemokine receptors on a human conjunctival cell line (Chang cells). Methods: Conjunctival cells from Chang cell line were investigated at baseline and after incubation with IFN-g at different concentrations (20 and 200 IU/ml) for 72 hours. These cells with or without IFN-g stimulation were stimulated by various human recombinant chemokines (six CC chemokines, SDF-1, RANTES, MIP-1a, Eotaxin, TARC, and MCP-1 ; two CXC chemokines, IP-10, IL-8 and the only CX3C chemokine, Fraktalkine). Cells were observed with a station Quanticell 700 which permits the monitoring of the cytosolic calcium during stimulation by chemokines. If the chemokine receptor is present and functional, then is observed a variation in cytosolic calcium. Three experiments were performed for each chemokine at least. Results: The receptor for SDF-1 was present and functional on our cellular model with 100 % of cells presenting calcium variation. Receptors for RANTES and Fraktalkine were present at a lower level with respectively 75 and 50% of the cells showing variation of cytosolic calcium after stimulation. There was no difference between cells at baseline and after treatment with IFN-g for these chemokines. We observed a mild effect of IP-10 on our cells when treated with 200 IU/ml of IFN-g. Conclusions: Our results suggest that CXCR-4 is present on Chang at baseline. These preliminary findings need more investigation such as in vivo expression of CXCR-4 and SDF-1 in the ocular surface. This chemokine is implicated in angiogenesis. The description of this system could be interesting in the understanding and treatment of corneal neovascularization.

Keywords: conjunctiva • inflammation • calcium 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×