Abstract
Abstract: :
Purpose: Previous studies of our laboratory had report a significant increase of LBP levels in aqueous humor of rabbits after endotoxin- Induced Uveitis (EIU). In this study we investigate the production of LBP by the Iris-Ciliary body in hiperlipidemic rabbits, after Iris-Ciliary body stimulation with LPS and cytokines. Methods: New Zealand White rabbits (3.5-4 Kg) were fed with 0.5% cholesterol diet dissolved in corn oil (HChR), 12.5% corn oil diet (CR) and normal rabbits (NR), for 8 weeks. After this period rabbits'Iris-Ciliary body were obtain under sterile conditions. Culture in RPMI 1640 supplemented with 1% antibiotic and 1% glutamine. This culture were prompted with LPS, cytokines such as IL-1, IL-6, TNF or combinations of this three cytokines. After 48 hours medium was harvested and LBP was mesured by an ELISA (Donated by Dr. Peter Tobias). In those cultures stimulated with LPS, we also measured IL-6 by B9 bioassay and TNF by WEHI 164 clone 13 bioassay. Results: LBP levels in explant supernatant of CR and NR prompted with 100ng/ml of IL-6 (26.13%, n=9) and (14.21%, n=9) respectively showed an statistical significant increase (p=0.05) and (p=0.04) respectively in comparison to their controls. IL-6 levels were significantly increased (p=0.05) in CR iris prompted with LPS 100ng/ml (15716.81%, n=9) in comparison with the not prompted controls. TNF was significantly increased (p= 0.03) in HChR Iris-Cilary body challenged with LPS 100ng/ml ( 274976%, n=8) and Iris-Ciliary body challenged with LPS 200ng/ml (p= 0.03) (117330%, n=8) in comparison to not challenged controls. Conclusions: This is the first report that indicates the producction of LBP by the Iris-Ciliary body. TNF and IL-6 were produced by the Iris-Ciliary body after LPS challenge. Our data suggest that cytokines induce LBP levels might be playing an important role in the eye inflamatory process.
Keywords: inflammation • iris • lipids