May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Studies of Monoclonal Antibody Against Retinoblastoma Cells and the Purification of Antigen
Y. Li; f. Ying; S. Tong; L. Liu; B. Ning
Author Affiliations & Notes
  • Y. Li
    Zhongshan Ophthalmic Center, Zhong Shan University, Guangzhou, China
  • f. Ying
    Zhongshan Ophthalmic Center, Zhong Shan University, Guangzhou, China
  • S. Tong
    Zhongshan Ophthalmic Center, Zhong Shan University, Guangzhou, China
  • L. Liu
    Zhongshan Ophthalmic Center, Zhong Shan University, Guangzhou, China
  • B. Ning
    Zhongshan Ophthalmic Center, Zhong Shan University, Guangzhou, China
  • Footnotes
    Commercial Relationships  Y. Li, None; F. Ying, None; S. Tong, None; L. Liu, None; B. Ning, None.
  • Footnotes
    Support  Grants 211, NNSFC 39870801, NSFGD 98011
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 759. doi:
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      Y. Li, f. Ying, S. Tong, L. Liu, B. Ning; Studies of Monoclonal Antibody Against Retinoblastoma Cells and the Purification of Antigen . Invest. Ophthalmol. Vis. Sci. 2003;44(13):759.

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Abstract

Abstract: : Purpose:To produce monoclonal antibody directed against tumor antigen of retinoblastoma-derived tissue culture cell line SO-Rb50 and purify the SO-Rb50 cells antigen corresponding to the monoclonal antibody of anti-restinoblastoma. Methods: Hybridization was performed and the specificity of the antibody was tested by immunofluorescent and immunohistochemical methods. The purification of the antigen was made using ion-exchange chromatography, DOT-BLOT and SDS-PAGE. Results:Two hybridomas secreted specific monoclonal antibody against retinoblastoma cells were produced,and the isotype of the antibody was IgG2a. After ion-exchange chromatography of SO-Rb50 cells antigen, SDS PAGE only showed a special 83kd unmixed band, stained by the antibody against retinoblastoma cell using WEST-BLOT. Conclusions: The monoclonal antibody is specific and highly active against retinoblastoma cells. The character of the antigen differ from Rb protein of Rb gene product.

Keywords: retinoblastoma • antigen presentation/processing 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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