May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
The Effect of Air Contact on MUC1 Expression in Rabbit Corneal Epithelial Culture
K. Jin; U.S. Kim; T.K. Park; J.M. Kim; J.K. Park
Author Affiliations & Notes
  • K. Jin
    Ophthalmology, Kyung Hee University, Seoul, Republic of Korea
  • U.S. Kim
    Ophthalmology, Kyung Hee University, Seoul, Republic of Korea
  • T.K. Park
    Ophthalmology, Soonchunhyang Univ Hospital, Bucheon, Republic of Korea
  • J.M. Kim
    Ophthalmology, Soonchunhyang Univ Hospital, Bucheon, Republic of Korea
  • J.K. Park
    Ophthalmology, Kyung Hee East-West Medical Research Institute, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  K. Jin, None; U.S. Kim, None; T.K. Park, None; J.M. Kim, None; J.K. Park, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 853. doi:
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      K. Jin, U.S. Kim, T.K. Park, J.M. Kim, J.K. Park; The Effect of Air Contact on MUC1 Expression in Rabbit Corneal Epithelial Culture . Invest. Ophthalmol. Vis. Sci. 2003;44(13):853.

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Abstract

Abstract: : Purpose: To investigate the effect of air-contact on MUC1 expression and epithelial differentiation in rabbit corneal epithelial cell culture. Methods: The rabbit corneal epithelial cells were cultured in air-contact culture system using multiwell plate membrane or conventional culture system for 5 days. The corneal epithelial cell differentiation was determined by hematoxylin-eosin stain. The MUC1 expression was determined by immunohistochemical stain using monoclonal anti-human milk fat globulin for MUC1 (HMFG-α). Results: The corneal epithelium was more differentiated in air-contact culture than conventional culture. MUC1 was detected at apical portion of air-contact cultured corneal epithelium in immunohistochemical stain, whereas there was no MUC1 staining on corneal epithelium in conventional culture. Conclusions: The air (possibly O2) influenced on the expression of MUC1 in cultured rabbit corneal epithelium and also affected the cellular differentiation of corneal epithelium

Keywords: cornea: epithelium • gene/expression • cornea: basic science 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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