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T. Miyamoto, S. Saika, T. Ueyama, Y. Okada, T. Sumioka, N. Fujita, A. Yamanaka, Y. Ohnishi; Expression of Heat-shock Proteins and HSPmRNAÅ@in Corneal Epithelium Following an Exposure to Ethanol . Invest. Ophthalmol. Vis. Sci. 2003;44(13):860.
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Purpose: To examine the expression pattern of heat-shock proteins (HSPs) and HSP mRNA following an ethanol exposure in rat and rabbit cornea to model a pre-treatment of LASEK.Methods: (1) Cornea of one eye of Wistar rats (n=22) was exposed to 20% ethanol (30 sec) and allowed to heal up to 48 hr. The animals were sacrificed and enucleated eye was subjected to in situ hybridization for mRNA of HSP 27, 70 and 90 by using radio-labeled probes. (2) Central cornea of one eye in adult albino rabbits (n=16) was subjected to an ethanol exposure (30%, 30 sec) and washed by saline. After healing intervals up to Day 7, the animals were killed and cornea was processed for immunohistochmeistry for HSP27, 60 and 90a.Results:Corneal epithelial cells were found to be alive after the ethanol exposure in rabbit and rat eyes. (1) In rats, HSP70 mRNA was expressed corneal epithelium during 1 - 6 hr post-treatment. mRNA expression of both HSP27 and HSP90 was delayed as compared to HSP70 expression and was detected from 3 to 12 hrs in epithelium post-treatment. In rabbit cornea, HSP27 was detected in epithelium and keratocytes form Day 0.5 - 1 after ethanol exposure. At Day 2 epithelium returned to be negative, but keratocytes were positive for HSP27. HSP60 or HSP90a was detected in epithelium and keratocytes at Day 0.5 ñ 3, or Day 0.5 - 2, respectively.Conclusions: Ethanol exposure induces expression of HSPs in corneal epithelium and/or keratocytes in rats and rabbits. HSPs up-regulation following ethanol exposure may have a role in cell survival following an ethanol exposure in both corneal epithelium and keratocytes.
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