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C. Choe, I. Lee, M. Jang, J. Heo; Effect of BMP 7 on Scarless Wound Healing . Invest. Ophthalmol. Vis. Sci. 2003;44(13):905.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose : To identify a scar formation inhibitory protein in human amniotic membrane (AM) and to evaluate the efficacy of the protein on corneal epithelial wound repair. Methods : AM was obtained from Korean healthy donor, and proteins from AM were fractionated and assessed for the ability to inhibit myofibroblast formation in HaCat cells. The fraction with the highest inhibitory effect was subjected to two-dimensional electrophoresis and the fraction with the highest inhibitory effect was subjected to two-dimensional electrophoresis, and each protein spot was analyzed by MALDI-TOF and ESI-TOF MS/MS. Identified proteins were purified and assayed for the effect on myofibroblast formation and scar formation in rat cornea. Result : Analysis of AM proteins by MALDI revealed BMP-7 is one of the major proteins. Purified BMP-7 was able to inhibit myofibroblast formation in HaCat cells. Addition of BMP-7 to HaCat cells was found to repress the fibronectin expression as confirmed by RT-PCR and western blot analysis. In addition, BMP-7 was found to suppress scar formation on rat cornea. Conclusion : We have identified BMP-7 from human AM and demonstrated that BMP-7 has an inhibitory effect on myofibroblast formation in cell culture and scar formation in an rat cornea. These data suggested a potential use of BMP-7 for scarless wound healing.
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