May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Circulating Antioxidants, Lipid Peroxidation Products, Cytokines and Neuron-specific Enolase in Leber’s Hereditary Optic Neuropathy (LHON) Patients
Author Affiliations & Notes
  • T. Lam
    Ophthalmology, Doheny Eye Institute, Los Angeles, CA, United States
  • V. Carelli
    Neuroscience, Univ. of Bologna, Bologna, Italy
  • S. Salomao
    Ophthalmology, Fed. Univ. of Sao Paolo, Sao Paolo, Brazil
  • R. Belfort
    Ophthalmology, Fed. Univ. of Sao Paolo, Sao Paolo, Brazil
  • A. Berezovsky
    Ophthalmology, Fed. Univ. of Sao Paolo, Sao Paolo, Brazil
  • P. Quiros
    Ophthalmology, Fed. Univ. of Sao Paolo, Sao Paolo, Brazil
  • F. Sadun
    Rome, Italy
  • A.M. DeNegri
    Rome, Italy
  • J. Sherman
    SUNY Col of Optometry, New York, NY, United States
  • A.A. Sadun
    SUNY Col of Optometry, New York, NY, United States
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 938. doi:
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      T. Lam, V. Carelli, S. Salomao, R. Belfort, A. Berezovsky, P. Quiros, F. Sadun, A.M. DeNegri, J. Sherman, A.A. Sadun; Circulating Antioxidants, Lipid Peroxidation Products, Cytokines and Neuron-specific Enolase in Leber’s Hereditary Optic Neuropathy (LHON) Patients . Invest. Ophthalmol. Vis. Sci. 2003;44(13):938.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To explore indicators of injury to the inner retina and optic nerve in LHON patients. Methods: Serum and plasma samples were collected from 43 subjects: 15 male affected, 6 non-affected male carriers, 5 normal male, 2 female affected, 11 female carriers, 4 normal female from a Brazilian population previously examined for LHON. All patients were part of a previously described giant Brazilian pedigree demonstrated by molecular analysis to be 11778 mtDNA with J-haplogroup. Commercially available assay kits were used to determine circulating levels of total antioxidant, lipid peroxidation products, interleukin-3 (IL-3), tumor necrosis factor – alpha (TNF-α) and neuron-specific enolase (NSE). Comprehensive ophthalmologic and neuro-ophthalmologic examinations were performed on these individuals. Results: There were no significant differences in circulating levels of total antioxidants, lipid peroxidation products, IL-3 and TNF-α. However, ANOVA test followed by Dunn’s pairwise test reviewed significant elevation of circulation NSE levels in male carriers when compared to the male normal controls. These carriers also showed evidence of subtle subclinical abnormalities such as Tritan color deficiency and prolonged latencies in pattern reversal VEP. Conclusions: NSE may be a good indicator of retinal ganglion cell and optic nerve stress/degeneration. There were no indications of oxidative stress and or cytokines upregulation, such as TNF-α and/or IL-3 as important factors for the development of visual impairment in LHON.

Keywords: clinical laboratory testing • degenerations/dystrophies • neuro-ophthalmology: optic nerve 
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