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M.K. Call, P.A. Tsonis; Studying the Ability for Lens Regeneration Through Cross-Species Transplantations . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1252.
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Purpose: To date it is not understood if the ability for lens regeneration is due to the intrinsic nature of the dorsal iris or to an external factor. Our goal was to delineate between these two possibilities by performing a series of cross-species transplantations. Methods: Cross transplantations were performed utilizing two species of urodeles, the newt (capable of lens regeneration) and the axolotl, which possess the ability to regenerate body parts such as the limb and tail but is not capable of lens regeneration. In these transplantations, dorsal iris explants or dorsal pigmented epithelial cell (PEC) reaggregates from the newt were placed into either the regenerating limb or lentectomized eye of the axolotl. The reverse was also performed taking axolotl iris explants and PEC reaggregates and placing them into the newt. Parallel experiments were also run where the tissue and cells were treated with retinoic acid, which has been shown to determine morphogenesis and differentiation of the eye and limb. Results: Our preliminary results show that the ability of the dorsal iris to transdifferentiate into lens is intrinsic to this tissue. The outcome of our results will be discussed in light of the necessity for extrinsic/intrinsic factors during the process of lens regeneration. Conclusions: These results no doubt further our understanding of the process of lens regeneration and provide a first step into understanding the restriction seen in other animals.
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