May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
In Vitro Epithelialization of a Synthetic Polymer for Generation of Corneal Onlay/keratoprosthesis
S.A. Pilyugina; A. Lavoie; P. Huie; K. Derr; A.J. Smith; J. Noolandi; R.M. Waymouth; C. Ta
Author Affiliations & Notes
  • S.A. Pilyugina
    Department of Ophthalmology, Stanford School of Medicine, Stanford, CA, United States
  • A. Lavoie
    Department of Chemistry, Stanford University, Stanford, CA, United States
  • P. Huie
    Department of Chemistry, Stanford University, Stanford, CA, United States
  • K. Derr
    Department of Chemistry, Stanford University, Stanford, CA, United States
  • A.J. Smith
    Department of Chemistry, Stanford University, Stanford, CA, United States
  • J. Noolandi
    Department of Chemistry, Stanford University, Stanford, CA, United States
  • R.M. Waymouth
    Department of Chemistry, Stanford University, Stanford, CA, United States
  • C. Ta
    Department of Chemistry, Stanford University, Stanford, CA, United States
  • Footnotes
    Commercial Relationships  S.A. Pilyugina, VISX, Inc. F; A. Lavoie, None; P. Huie, VISX, Inc. F; K. Derr, VISX, Inc. F; A.J. Smith, None; J. Noolandi, VISX, Inc. F, P; R.M. Waymouth, None; C. Ta, VISX, Inc. F, P.
  • Footnotes
    Support  VISX Inc.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1344. doi:
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      S.A. Pilyugina, A. Lavoie, P. Huie, K. Derr, A.J. Smith, J. Noolandi, R.M. Waymouth, C. Ta; In Vitro Epithelialization of a Synthetic Polymer for Generation of Corneal Onlay/keratoprosthesis . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1344.

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Abstract

Abstract: : Purpose: To achieve epithelialization of a collagen I-coated synthetic polymer surface. Methods: Poly (2-hydroxyethyl methacrylate) (PHEMA) hydrogel lenticules (synthesized within our laboratories; composition: 6 % methacrylic acid, 93 % HEMA, and 1% EGDMA = ethyleneglycol dimethacrylate) and commercially available Vifilcon-A® contact lenses (American Optical; composition: HEMA and PVP = poly (vinyl pyrrolidone)) were used as test materials. Test surfaces were modified to aldehyde groups by TEMPO/H2O2 oxidation and subsequently functionalized with collagen I. Non-modified contact lens and polymer lenticules served as controls. Surface epithelialization was tested in vitro using a bovine corneal organ culture model: corneal wound was induced by debriedment of central corneal epithelium. Test lenticules (8 mm diameter ) were implanted into a corneal stromal pocket and maintained in air/liquid organ culture system. Epithelialization rate was monitored clinically using fluorescein over a period of 1 week. Epithelial architecture was examined histologically by light microscopy. Electron microscopy was used to examine the tissue-polymer interface for evidence of hemidesmosomal adhesion complex formation. Results: Clinical observation of the epithelialization process demonstrated complete re-epithelialization of the sham wound and collagen I-coated modified contact lens by Day 5. Collagen I-coated PHEMA lenticule re-epithelialized at a slower rate by Day 7. Aldehyde-modified contact lens (no collagen I present) only partially re-epithelialized by Day 7. Non-modified PHEMA lenticule and contact lens did not exhibit any epithelial overgrowth. Histological evaluation of the sham wound and collagen I-coated contact lens revealed the presence of architecturally-intact epithelium with a basal cell layer and 2-3 layers of epithelial cells. Aldehyde-modified contact lens demonstrated a single layer of epithelial cells, with no evidence of basal cell differentiation. Conclusions: Collagen I-coated PHEMA hydrogel surface appears to support epithelialization in vitro. This polymer shows potential for further use in development of a corneal onlay or keratoprosthesis device that requires epithelialization.

Keywords: keratoprostheses • cornea: epithelium • wound healing 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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