The Use of Autologous Platelet Gel (Platelet-Rich Plasma) in Corneal Limbal Epithelial Cell Transplantation

Abstract

Abstract: : Purpose: Transplantation of corneal limbal epithelial cells is a promising treatment for total limbal stem cells deficiency (LSCD). However, the surgical technique is not easy. The sheet to be transplanted is delicate and thin. Suture is rather difficult to perform and it has been pointed out as a risk factor for blood vessels growth into the cornea. To overcome these difficulties we aimed to demonstrate the efficiency of an autologous platelet gel (platelet-rich plasma) to attach the sheet of limbal epithelial cells on the surface of the host cornea. Methods: Left eyes of 4 rabbits were rendered total LSCD by 1NNaOH on the entire corneal epithelium. At 8 weeks after the ocular surface injury, the fibrovascular pannus was surgically removed followed by the transplantation of cultivated limbal stem cells. This corneal sheet was 6 millimeters bigger in diameter than that of the host cornea. The platelet gel was put onto the sclera three millimeters sorrounding the cornea, and the corneal sheet was gently placed on the corneal and scleral surface. Results: One week after surgery the four transplants showed a successful attachment. The usual inflammatory reaction was seen. Conclusions: It is worthwhile to evaluate the effect of autologous platelet gel in this kind of surgery in a longer follow-up. The use of platelet gel may be a valuable tool for limbal epithelial cell transplantation.

Keywords: cornea: epithelium • transplantation • cornea: stroma and keratocytes