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M. Fukuda, H. Murano, Y. Yamashiro, N. Takahashi; Effects of Povidone Iodine on Cultured Corneal Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1366.
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Purpose: Povidone-iodine(PVP-I) is a broad-spectrum microbiocide with in vitro activity against gram-negative and gram-positive bacteria, fungi and protozoa. PVP-I is used for disinfection of palpebral skin and the conjunctival sac in ophthalmic surgery, but it induces cytotoxicity corneal tissue. In this study, the cytotoxicity of PVP-I was examined and expressed quantitatively and morphologically using cultured rabbit corneal epithelial cell lines (SIRC). Methods: We used 10% (w/v) PVP-I(Isodine ® solution) in this study. Cultured SIRC cells were exposed to PVP-I diluted at 0.312% for 30 seconds to 4 minutes, and at 0.1~0.5% for 30 seconds. The cytotoxicity of PVP-I was also microscopically (light microscopy and transmission electron microscopy ) observed after a 30 second exposure of SIRC cells to diluted PVP-I. Results: Treatment of SIRC cells with PVP-I diluted at 0.312% for 30 seconds to 4 minutes induced decrease of the cells in a treatment time-dependent manner. Exposure of cells to PVP-I at 0.1~0.5% for 30 secounds also caused decrease of the cells in a dose-dependent manner (50%viability ratio;VR-50:0.312%). On the other hand, cytotoxicity of PVP-I was microscopically observed after a 30 secound exposure of SIRC cells to PVP-I and necrosis predominated in cells treated with PVP-I solution. Conclusions: These results indicate that cytotoxicity of PVP-I occurs in dose-and treatment time-dependent manners. Microscopically, the necrosis cells predominates in cells that have been treated with PVP-I solutions. A 0.312% solution of PVP-I should be used in ocular disinfection for less than 30 seconds.
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