May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Adeno-associated Delivery of TIMP-3 Inhibits Experimental Choroidal Neovascularization
K.C. Behling; A. Kuroki; J. Fosnot; S.J. Reich; A.M. Maguire; M.J. Tolentino; J. Bennett
Author Affiliations & Notes
  • K.C. Behling
    Department of Ophthalmology, University of Pennsylvania, FM Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, Philadelphia, PA, United States
  • A. Kuroki
    Department of Ophthalmology, University of Pennsylvania, FM Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, Philadelphia, PA, United States
  • J. Fosnot
    Department of Ophthalmology, University of Pennsylvania, FM Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, Philadelphia, PA, United States
  • S.J. Reich
    Department of Ophthalmology, University of Pennsylvania, FM Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, Philadelphia, PA, United States
  • A.M. Maguire
    Department of Ophthalmology, University of Pennsylvania, FM Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, Philadelphia, PA, United States
  • M.J. Tolentino
    Department of Ophthalmology, University of Pennsylvania, FM Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, Philadelphia, PA, United States
  • J. Bennett
    Department of Ophthalmology, University of Pennsylvania, FM Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, Philadelphia, PA, United States
  • Footnotes
    Commercial Relationships  K.C. Behling, None; A. Kuroki, None; J. Fosnot, None; S.J. Reich, None; A.M. Maguire, None; M.J. Tolentino, None; J. Bennett, None.
  • Footnotes
    Support  NIH EY1341 and EY12156, RPB, IRRF, JDRF, FM Kirby Foundation
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1425. doi:
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      K.C. Behling, A. Kuroki, J. Fosnot, S.J. Reich, A.M. Maguire, M.J. Tolentino, J. Bennett; Adeno-associated Delivery of TIMP-3 Inhibits Experimental Choroidal Neovascularization . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1425.

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Abstract

Abstract: : Purpose: These experiments aim to determine if AAV2/1-mediated delivery of tissue inhibitor of metalloproteinase-3 (TIMP3) can inhibit experimental laser induced choroidal neovascularization (CNV) in mice. Methods: AAV2/1-CMV-TIMP3 was delivered unilaterally to adult female C57BL/6 mice via subretinal injections. Contralateral eyes received subretinal injections of AAV2/1-CMV-LacZ and served as controls. Three weeks post-injection, mice underwent laser photocoagulation using an 810 nm diode laser (OcuLight Six; IRIS Medical, Mountain View, CA). Three laser spots were applied in both eyes. Two weeks after laser treatment, animals were perfused with high molecular weight FITC-Dextran, and choroidal flat mounts were prepared and analyzed microscopically. The area of CNV was measured with Openlab software (Improvision, Boston, MA) in a masked fashion. Results: Flat mount analysis revealed a statistically significant decrease in CNV in AAV2/1-CMV-TIMP3-treated eyes when compared to controls. Conclusion: AAV2/1-CMV-TIMP3 can reduce neovascularization in a murine model of laser induced CNV and may be a promising method of inhibiting CNV in humans.

Keywords: choroid: neovascularization • gene transfer/gene therapy • animal model 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2023 Association for Research in Vision and Ophthalmology.
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