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V.H. Karageszian, H.L. Karageozian, A. Climent, S. Munoz, G. Cuevas, V.G. Gallegos; Determine the In-vitro Antimicrobial Preservative Effectiveness of a New Broad Spectrum Antimicrobial Preservative "Oxyd" . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1469.
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Purpose: To evaluate the in-vitro antimicrobial preservativeeffectiveness of a new broad spectrum antimicrobial preservative"Oxyd" in an Ophthalmic tear solution.Methods: The preservativeeffectiveness test methodology of the United States Pharmacopeia25 (USP) was used in all the in-vitro testing Working culturesof ATCC strains of P.aeruginosa, S.aureus, E.coli, C. albicansand A. niger, or human resistant strains were prepared as perthe USP. Sterile saline suspensions of the five organisms areprepared in separate sterile culture tubes, to contain 106 -108 colony forming units/ mL. of solution. 20 mL. of productsample is transferred aseptically to 5 sterile capped bacteriologicaltubes, Each product tube is inoculated with one of the standardizedmicrobial suspensions, using a ratio equivalent to 0.10 mL.of inoculum to 20 mL. of product. and mixed. The number of viablemicroorganisms in each inoculum suspension is determined bythe plate-count method. The inoculated containers are incubatedat 20° - 25°. The culture tubes are read at 1, 2, 3,4, 6 and 24 hours,7 and 14 days.Results: View OriginalDownload SlideView OriginalDownload Slide View OriginalDownload SlideView OriginalDownload SlideConclusions: "Oxyd" the new antimicrobial preservative is veryeffective as a broad spectrum antimicrobial agent.
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