May 2003
Volume 44, Issue 13
ARVO Annual Meeting Abstract  |   May 2003
Localization of RPGR to Centrosomes
Author Affiliations & Notes
  • F.D. Manson
    Academic Department of Ophthalmology, University of Manchester, Manchester, United Kingdom
  • A.J. Faragher
    Department of Biochemistry, University of Leicester, Leicester, United Kingdom
  • B. Tulloch
    Cell and Molecular Genetics, MRC Human Genetics Unit, Edinburgh, United Kingdom
  • A.M. Fry
    Cell and Molecular Genetics, MRC Human Genetics Unit, Edinburgh, United Kingdom
  • A.F. Wright
    Cell and Molecular Genetics, MRC Human Genetics Unit, Edinburgh, United Kingdom
  • Footnotes
    Commercial Relationships  F.D.C. Manson, None; A.J. Faragher, None; B. Tulloch, None; A.M. Fry, None; A.F. Wright, None.
  • Footnotes
    Support  Foundation Fighting Blindness
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1530. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      F.D. Manson, A.J. Faragher, B. Tulloch, A.M. Fry, A.F. Wright; Localization of RPGR to Centrosomes . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1530.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: To further characterize the intracellular localization of RPGR. Methods: Native RPGR was localised by immunofluorescence in three human cell lines (HeLa, U2OS, ARPE19) and in human retina using four different antibodies raised against synthetic peptides or expressed recombinant peptide. Cells were also labelled with markers for centrosomes, Golgi and spliceosomes. Results: With all four antibodies, punctate nuclear labelling was found in each cultured cell type and in all retinal layers, including the RPE. Discrete localisation was also observed outside of the nucleus in interphase cells, similar to that seen with pericentrin, using antibodies against the C-terminal of ORF15. This labelling co-localised with gamma-tubulin, a marker for the centrosome. Weaker spindle pole labelling was observed in mitotic cells. Co-localisation was not observed with markers for either the Golgi or spliceosome. Pre-immune sera gave no labelling. Conclusions: Native RPGR has a dual intracellular localisation in cultured cells. It is present in the nucleus and also in the cytoplasm where it co-localises with gamma-tubulin in metaphase and mitotic cells. This localisation indicates that RPGR is associated with the centrosome. RPGR may participate, directly or indirectly, in the synthesis or function of microtubules.

Keywords: proteins encoded by disease genes • immunohistochemistry • retinal degenerations: cell biology 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.