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N. Ardjomand, A.M. Theissl, J. Faulborn; Paraffin Embedding Technique for Specimens Obtained by Vitrectomy . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1592.
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Purpose: Histological and immunohistochemical evaluation of vitrectomized specimens is challanging, since paraffin embedding and long time storage of such a material is not possible. In this study we introduce two different techniques for paraffin embedding of vitrectomized specimens. Methods: Vitrectomized material was collected in 123 cases. All materials were centrifuged at 2000 rpm for 10 minutes. All materials were embedded in paraffin via thromboplastin or celloidin. Results: Sections could be performed with both techniques down to 4µm. Histologic and immunohistochemical evaluation showed similar results with both techniques. Conclusions: Paraffin embedding and immunohistochemical staining of vitrectomized material is possible via thromboplastin or celloidin and offers excelent morphology. In contrast to the celloidin technique, which takes two weeks of preparation, the thromboplastin technique can be performed within 48 hours. Therefore we recommend paraffin embedding of vitrectomized specimens via thromboplastin for routine histologic preparation.
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