May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Oxidant Stress Inhibits PDGF-Induced RPE Migration
Y. Jiang; S. He; M. Jin; S.J. Ryan; D.R. Hinton
Author Affiliations & Notes
  • Y. Jiang
    Ophthalmology, Doheny Vision Research Ctr, Los Angeles, CA, United States
  • S. He
    Ophthalmology, Doheny Vision Research Ctr, Los Angeles, CA, United States
  • M. Jin
    Ophthalmology, Doheny Vision Research Ctr, Los Angeles, CA, United States
  • S.J. Ryan
    Ophthalmology, Doheny Vision Research Ctr, Los Angeles, CA, United States
  • D.R. Hinton
    Pathology, Doheny Vision Research Ctr, Los Angeles, CA, United States
  • Footnotes
    Commercial Relationships  Y. Jiang, None; S. He, None; M. Jin, None; S.J. Ryan, None; D.R. Hinton, None.
  • Footnotes
    Support  NIH grants EY02061,EY03040;Unrestricted grant from RPB; Arnold Mabel Beckman Foundation
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1633. doi:
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      Y. Jiang, S. He, M. Jin, S.J. Ryan, D.R. Hinton; Oxidant Stress Inhibits PDGF-Induced RPE Migration . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1633.

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Abstract

Abstract: : Purpose: Retinal pigment epithelial cells (RPE) undergo functional and morphological changes in response to oxidant stress. This study sought to determine if platelet derived growth factor (PDGF)-induced RPE migration could be modulated by oxidant stress induced by tert-butyl hydroperoxide (tbh). Methods: Cell migration was tested using passage 2-4 human RPE cells in a modified Boyden Chamber Assay. Cells were treated with different concentrations of tbh (10-200uM) for one hour, and chemotaxis toward PDGF (30ng/ml) was measured after an additional 5 hours. Changes in the actin cytoskeleton were evaluated microscopically with phylloidin staining. Effects of tbh on RPE cell survival were determined using the MTT assay and trypan blue staining. Results:: RPE cell migration induced by PDGF was significantly inhibited by pretreatment with tbh at a dose of 50uM and above. F-actin was aggregated in the periphery of the cells after treatment with tbh at a dose higher than 100uM. MTT assay and trypan blue staining showed no evidence of cell death during the 6 hours of this assay. When cells were maintained for 16 hours, cell death occurs with % survival ranging from 95.74 % (50 uM) to 44.44% (200 uM). Suprisingly, PDGF protected RPE cells from tbh (50 uM) -induced cell death. Conclusions: Oxidant stress induced with tbh can inhibit PDGF-induced RPE cell migration. CR: None

Keywords: age-related macular degeneration • oxidation/oxidative or free radical damage • retinal pigment epithelium 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2015 Association for Research in Vision and Ophthalmology.
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