May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Effect of in vivo Electroporation on Rat Retina
A. Uemura; A. Mizota; E. Sato; X. Mo; E. Adachi-Usami
Author Affiliations & Notes
  • A. Uemura
    Dept.of Ophth and Visual Science, Graduate School of Medicine, Chiba university, Chiba city, Japan
  • A. Mizota
    Dept.of Ophth and Visual Science, Graduate School of Medicine, Chiba university, Chiba city, Japan
  • E. Sato
    Dept.of Ophth and Visual Science, Graduate School of Medicine, Chiba university, Chiba city, Japan
  • X. Mo
    Dept.of Ophth and Visual Science, Graduate School of Medicine, Chiba university, Chiba city, Japan
  • E. Adachi-Usami
    Dept.of Ophth and Visual Science, Graduate School of Medicine, Chiba university, Chiba city, Japan
  • Footnotes
    Commercial Relationships  A. Uemura, None; A. Mizota, None; E. Sato, None; X. Mo, None; E. Adachi-Usami, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1904. doi:
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      A. Uemura, A. Mizota, E. Sato, X. Mo, E. Adachi-Usami; Effect of in vivo Electroporation on Rat Retina . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1904.

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Abstract

Abstract: : Purpose:We have used electroporation to transfer the heat shock protein and brain derived neurotorophic factor genes into retinal ganglion cells of rats. The purpose of this study was to determine the effect of electroporation on the rat retina as assessed by ERGs and morphological studies. Methods:Under deep anesthesia, right eye of 8-week-old Wistar rats was grasped between forcep-type electrodes, with the cathode attached to the corneal and anode attached to scleral surface. Single electric pulse with electric field strengths of 10, 20, 30, 40, and 50V/cm were delivered. The pulse was square wave modulated, and the duration was 99 ms. ERGs were recorded before and one week after the electroporation. The rats were sacrificed after the ERGs, and the eyes were enucleated for histopathological study. The number of retinal ganglion cells were counted, and anterior segments were observed by microscopy. Results:Corneas of the rat that underwent electroporation with electric field strength of >30 V/cm showed thermal burns. Electric field strength of >20 V/cm, the amplitude of both a- and b-wave on ERGs and the number of retinal ganglion cells decreased significantly. Conclusions:These findings suggest that electroporation with an electric field strength of 10 V/cm or less does not affect the retina and cornea significantly in rats.

Keywords: ganglion cells • electroretinography: non-clinical • anterior segment 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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