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M. Javaheri, G.Y. Fujii, J.V. Rossi, C.Q. Panzan, D. Yanai, D. Guven, M. Maia, E. de Juan, Jr., M.S. Humayun; The Effects of Various Intraocular Irrigating Solutions on the Rabbit Electroretinogram . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1905.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: A pilot study of varying combinations of intraocular irrigating solutions was undertaken in rabbits to investigate the effect of these factors on the electroretinogram (ERG) of post-vitrectomy subjects. Methods: Eight groups, each consisting of four to five rabbits, were selected and pars plana vitrectomy was performed on the right eye of each rabbit with the other serving as control. The components of the intraocular irrigating solutions were varied per each group: Balanced Salt Solution (BSS), BSS-plus® (BSSp), and combinations of each with infused oxygen (O2) and/or light (L). Infusion fluids were kept at room temperature in each experimental group. The subjects were evaluated by single flash ERG's under scotopic conditions, comparing the b-wave amplitudes of the surgical eye relative to the control, at pre-surgery, one hour, one day, one week and one month post-operatively. Results: BSSp+O2 (0.69), BSS+O2+L (0.66), and BSS+O2 (0.62) exhibited the fastest return to near-baseline amplitude ERG values at one hour post-op. At one-hour, BSS+O2 exhibited a significantly faster return to baseline when compared to both BSS (p=0.04) and BSS+L (p=0.02). BSS+L+O2 also exhibited a significantly faster return to baseline when compared to both BSS (p=0.002) and BSS+L (p=0.005). BSS+O2 showed a faster return to baseline (0.62 vs. 0.50) relative to BSSp, but was not significant (p=0.09). BSSp+O2 (0.95), BSS+O2 (0.92), and BSSp (0.95) exhibited the fastest return to near-baseline amplitude ERG values at one-day post op. At one day, BSS+O2 showed a significantly faster return to baseline when compared to BSS (p=0.04), while BSSp+O2 also showed a significantly faster return to baseline when compared to BSSp+L (p=0.003). BSS+O2 showed a slightly faster return to baseline than that of BSSp but these results were not significant (p=0.91). At later timepoints, values approached baseline. Conclusion: Evaluation of various intraocular irrigating solutions was significant (p<0.05) for faster short-term ERG recovery for BSS+O2 relative to BSS, with similar recovery patterns for BSSp and BSS+O2, indicating that this combination has significantly lower short-term ischemic effects on the retina during the procedure. This data provides some evidence for the use of oxygenated infusion fluids in vitreoretinal surgery with the prospect of decreasing short term postoperative recovery and reducing the cost of patient care.
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