May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
The Cux-1 Transgenic Mice Display Reduced Retinal Vascular Density and Undergo Retinal Degeneration
Author Affiliations & Notes
  • S. Wang
    Ophthalmology, University of Wisconsin Medical School, Madison, WI, United States
  • Z. Wu
    Ophthalmology, University of Wisconsin Medical School, Madison, WI, United States
  • J.G. Brantley
    Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS, United States
  • G.B. Vanden Heuvel
    Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS, United States
  • N. Sheibani
    Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS, United States
  • Footnotes
    Commercial Relationships  S. Wang, None; Z. Wu, None; J.G. Brantley, None; G.B. Vanden Heuvel, None; N. Sheibani, None.
  • Footnotes
    Support  Research to Prevent Blindness
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 2036. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      S. Wang, Z. Wu, J.G. Brantley, G.B. Vanden Heuvel, N. Sheibani; The Cux-1 Transgenic Mice Display Reduced Retinal Vascular Density and Undergo Retinal Degeneration . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2036.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Deregulated expression of the homeobox gene Cux-1 in transgenic mice results in multiorgan hyperplasia through down-regulation of the cyclin kinase inhibitor, p27Kip1, expression. This report investigates the role of Cux-1 expression on retinal development and vascularization. Methods: Retinal morphology was examined by preparation of histological sections from three-weeks and six-weeks old mice. Retinal vessels and vascular density were assessed utilizing retinal trypsin digests. The vascular endothelial cell, pericytes, and astrocytes were detected by staining wholemount retinas with antibodies to PECAM-1, NG-2, and glial fibrillary acidic protein (GFAP), respectively. Results: Histological examination showed total loss of photoreceptors throughout the retina in Cux-1 transgenic mice by six-weeks of age. The retinal trypsin digests demonstrated a lower retinal vascular density and thinner arterioles in Cux-1 transgenic mice compared to the wild type mice. GFAP staining of retina wholemounts revealed increased number of astrocytes in Cux-1 transgenic mice. Conclusion: We demonstrate that Cux-1 is an important regulator of retinal development and vascularization and its aberrant expression results in vascular abnormalities and retinal degeneration.

Keywords: retinal degenerations: cell biology • retinal development • retinal glia 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×