Abstract
Abstract: :
Purpose: To analyse the orbital fat after evisceration and enucleation according to the size and density of the adipocytes. Methods: This experimental study comprehended 13 specimens of 13 rabbits with 42 days-old submitted to socket surgery (evisceration and enucleation, 2 of whom were controls not submitted to surgery). Sacrifice of the animals were done at the 90th and at the 180th post-op days. A fragment of the orbital fat tissue were removed and fixed in formaline 10%, and underwent histologic evaluation with hematoxylin-eosin. Specimens were assessed by optic microscope (magnification: 100X) and 1000 µ2 area was delimited to study the fat cells present. The morfological parameters (number of cells and area of each cell) were analysed by Ipwin 32 software program and the groups (enucleation, evisceration and control group) were compared. Data were submitted to non-parametrical statistic test of Kruskall -Wallis (p>0,05). Results: There were no statistical differences among evisceration, enucleation or control group considering the mean area of adipocytes and density of cells per area. Conclusion: These findings show that atrophy of orbital fat tissue is not the main cause of orbital volume deficiency in the Anophthalmic Socket Syndrome.
Keywords: animal model • orbit • image processing