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A. Wang, H. Yu, Y. Wang, P. Hsieh, M. Fann; Cloning of a Novel Gene from the Subtractive Retinal cDNA Library following Optic Nerve Injury . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2261.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To clone and characterize a novel gene which was found to be up-regulated in the retina following optic nerve injury. Methods:Up-regulation of this novel gene was found in the subtractive hybridization and verified by RT-PCR. The mRNA and peptide sequences of this novel gene was determined using RT-PCR, TA cloning, sequencing, and bioinformatics. RNA expression profile was examined in adult mice tissue using Northern blot. The protein expression of this novel gene was investigated by immunostaining and Western blot. Results:Sequencing and bioinformatics revealed this novel gene to be a 5-kb transcript, which was confirmed on Northern blot. Predicted protein sequence and Western blot show a 65 kD peptide with a FERM domain. The immunostaining demonstrated that this novel gene is expressed on the retinal ganglion layer and implicated a cell-cell contact function of this gene. Conclusions:A novel gene was cloned and characterized from a subtractive retinal cDNA library following optic nerve injury.
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