May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
A Rat Model of Neurotrophic Keratitis: Anatomical and Physiological Studies
Author Affiliations & Notes
  • V. Jain
    Ophthalmology, Univ California-Irvine, Irvine, CA, United States
  • E.K. Wong Jr.
    Ophthalmology, Univ California-Irvine, Irvine, CA, United States
  • H. Agnic
    Ophthalmology, Univ California-Irvine, Irvine, CA, United States
  • S. Vong
    Ophthalmology, Univ California-Irvine, Irvine, CA, United States
  • E. Kang
    Ophthalmology, Univ California-Irvine, Irvine, CA, United States
  • C. Lee
    Ophthalmology, Univ California-Irvine, Irvine, CA, United States
  • A. Nafissi
    Ophthalmology, Univ California-Irvine, Irvine, CA, United States
  • J. Soriano
    Ophthalmology, Univ California-Irvine, Irvine, CA, United States
  • Y. Pham
    Ophthalmology, Univ California-Irvine, Irvine, CA, United States
  • R. Kinyamu
    Neuroscience, Univ California-Irvine, Irvine, CA, United States
  • Footnotes
    Commercial Relationships  V. Jain, None; E.K. Wong Jr., None; H. Agnic, None; S. Vong, None; E. Kang, None; C. Lee, None; A. Nafissi, None; J. Soriano, None; Y. Pham, None; R. Kinyamu, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 2445. doi:
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    • Get Citation

      V. Jain, E.K. Wong Jr., H. Agnic, S. Vong, E. Kang, C. Lee, A. Nafissi, J. Soriano, Y. Pham, R. Kinyamu; A Rat Model of Neurotrophic Keratitis: Anatomical and Physiological Studies . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2445.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Our research is focused on creating a reproducible model of Neurotrophic Keratitis (NK), a result of sensory denervation of the cornea via the ophthalmic branch of the trigeminal nerve (V1). The literature shows that an animal model is needed to study the efficacy of therapies and pathophysiology of this disease. Methods: To denervate the cornea we create a lesion in V1 using neurosurgical radiofrequency thermocautery (Radionics, #RFG-3C) administered through a stereotactic instrument with the animal lying supine. Recently, we have begun stimulating the nerve with up to 0.20 volts before creating the lesion. To further clarify the anatomy of the branching of the trigeminal nerve (CN V), we performed 25 microdissections of Sprague-Dawley rats between 190-430g.  

Keywords: animal model • keratitis • comparative anatomy 
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