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I. Kovacs, I. Lukacs, A. Ludany, J. Feher, B. Kovacs, J. Szolcsanyi, Ophthalmic Neuroscience Program; Changes in Tear Composition after Antidromic Electric Stimulation of Rat's Trigeminal Ganglia . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2529.
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Purpose: In our previous studies we presented clinical and morphological evidences that antidromic stimulation of rat's trigeminal ganglia influences accessory lacrimal gland, goblet cell and Meibomian gland functions. The parasympathetic pathway was not involved, but it is thought to be a direct influence of pro-inflammatory neuropeptides released from sensory nerve terminals. The aim of the present study was to test quantitative and qualitative changes of tear secretion caused by antidromic electric stimulation of trigeminal ganglia, i.e effects of neurogen inflammation on tear composition. Methods:These studies were carried out on male Wistar rats weigthing 250-350 g. The experimental protocol was identical to that of the previous studies on antidromic electric stimulation of rat's trigeminal ganglia. Tear samples were obtained by capillary tubes and they were studies using sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS – PAGE), and two dimensional polyacrylamide gel electrophoresis (PAGE). Results: Antidromic electrical stimulation of rat's trigeminal ganglia significantly increases tear volume. This increase comes primarily from higher amount of aqueous tear and from excessive release of mucus. However, stimulated tears also contain significant amount of plasma proteins, as increased plasma leakage from conjunctival (and glandular) vessels take place in neurogen inflammation. In addition, with the use of monoclonal antibodies against various tear components (lysozim, lactoferrin, MUC-1 and others) we were able to detected further differences in the amount of these proteins secreted into the tears at the stimulated side. Discussion: These results confirm our previous clinical and histological findings that antidromic electrical stimulation of rat's trigeminal ganglia resulted in increased release of tear constituents, which are different qualitatively from those of the unstimulated tears.
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