Purchase this article with an account.
B. Lu, N. Cuenca, S. Wang, H. Kolb, R.D. Lund; Morphological Changes in the Inner Retina of P23H Rhodopsin Transgenic Rat with the Progress of the Retinal Degeneration . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2833.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: To examine the effects of photoreceptor degeneration on the changes of inner retinal neurons in P23H rhodopsin transgenic rat. Methods: P23H heterozygote (line 1) and Sprague-Dawley (SD) control rats were studied at 3 weeks to 9months old by immunocytochemistry. A range of cell-specific antibodies was used including to rhodopsin, calcium binding proteins (calretinin, calbindin and parvalbumin), protein kinase C (α-PKC) and neurofilament (RT97). Retinal sections were examined by confocal microscopy. Results: Immunostaining showed that there are major morphological changes in the inner retina neurons as the progress of photoreceptor degeneration in P23H rats compared with normal control SD rats. Most photoreceptors are lost by 5 month of age as indicated by rhodopsin staining. Morphological changes began in the secondary neurons, even at 40 days. The changes in inner retinal neurons included: 1). Abnormal sprouting of horizontal cell dendrites (as indicated by RT97 and calbindin antibody staining). 2). Progressive loss of rod bipolar cell dendrites and reduction of their axon terminal size ( PKC antibody staining). 3). Loss of AII amacrine cell dendrites and lobular appendages. 4). Changes in expression pattern of calcium binding proteins: an increase in numbers of amacrines cells expressing calretinin and horizontal cells expressing calbindin; a decrease of AII amacrine cells expressing parvalbumin. Conclusions: This study revealed that there is early secondary pathology in the inner retina neurons paralleling primary photoreceptor loss in the P23H rat.
This PDF is available to Subscribers Only