May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Interleukin-10 (IL-10) after Ischemia Reperfusion Injury to the Rat Retina
Author Affiliations & Notes
  • R. Garg
    Ophthalmology, USC/Doheny Eye Inst, Los Angeles, CA, United States
  • R. Sanchez
    Ophthalmology, USC/Doheny Eye Inst, Los Angeles, CA, United States
  • C.K. Chan
    Ophthalmology, USC/Doheny Eye Inst, Los Angeles, CA, United States
  • S. Garg
    Ophthalmology, USC/Doheny Eye Inst, Los Angeles, CA, United States
  • M.J. Wong
    Ophthalmology, USC/Doheny Eye Inst, Los Angeles, CA, United States
  • A.A. Sadun
    Ophthalmology, USC/Doheny Eye Inst, Los Angeles, CA, United States
  • T.T. Lam
    Ophthalmology, USC/Doheny Eye Inst, Los Angeles, CA, United States
  • Footnotes
    Commercial Relationships  R. Garg, None; R. Sanchez, None; C.K. Chan, None; S. Garg, None; M.J. Wong, None; A.A. Sadun, None; T.T. Lam, None.
  • Footnotes
    Support  EY03040, Unrestricted Grant from RPB
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 2930. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      R. Garg, R. Sanchez, C.K. Chan, S. Garg, M.J. Wong, A.A. Sadun, T.T. Lam; Interleukin-10 (IL-10) after Ischemia Reperfusion Injury to the Rat Retina . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2930.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Introduction: We have previously showed that IL-10 changes after ischemia-reperfusion (I/R) injury to the rat retina. To further characterize the role of IL-10 after I/R injury, we localized IL-10 and evaluated the possible neuroprotective effect of IL-10 in the retina. Methods: Cannulas, connected to a saline column that generated a pressure of 110 mmHg, were inserted into the anterior chambers of Adult Lewis albino rats. The elevated pressure was maintained for 1 hour to render the retina ischemic and reperfusion was established after the ischemic period. Groups of animals were then euthanized at 0, 4, 12, 18 or 48 hours post reperfusion and their eyes were enucleated. The retinas were fixed, processed, and embedded in paraffin for sectioning. Immunohistochemistry was performed using anti-IL-10 (n=3) antibody. Other groups of animals were subjected to I/R injury and received intravitreal injections of 0 (n=5), 1 (n=4), 3 (n=7), 10 (n=8) or 30 (n=7) ng IL-10. These animals were euthanized 7 days after I/R injury, their retinas were flat mounted and the number of RGC’s was counted. Results: There was very little IL-10 immunoreactivity in normal retinas. However, IL-10 was localized to cells that resembled microglia in the inner nuclear layer at 18 and 48 hours after I/R injury. Unexpectedly, our pharmacologic study showed that intravitreal administration of IL-10 had no significant effect on the number of RGC’s when compared to vehicle treated retinas. Conclusion: There is IL-10 immunoreactivity in microglia-like cells in the inner retina at a late stage after I/R injury. Its role in neuroprotection/neurodegeneration is not clear and remains to be further examined.

Keywords: cytokines/chemokines • ischemia • neuroprotection 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×