Purchase this article with an account.
M. Hollborn, P. Wiedemann, L. Kohen; Cytokine Influence on the mRNA of Cell Cycle and ECM Proteins by hRPE Cells In vitro . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2948.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: The retinal pigment epithelial cells (RPE) play a pivotal role in the pathogenesis of the proliferative vitreoretinopathy (PVR). Changes of the concentrations of growth factors involved in the pathogenesis of PVR seem to induce the re-entering of different retinal cell types into the cell cycle. The aim of these investigations was to analyse the effects of growth factors on the proliferation of hRPE cells and on the mRNA expression of transcription factors, cell cycle proteins and extracellular matrix (ECM) proteins. Methods: Human RPE cells were incubated in the presence of TGFß1, TGFß2, PDGF, VEGF or bFGF and cell proliferation was determined by the BrdU incorporation after 24 - 72h. The changes in mRNA expression of c-fos, c-myc, PCNA, FEN1, Ki67, collagen III and collagen IV were investigated using the ribonuclease protection assay (RPA). Results: RPE cell proliferation was significant increased by PDGF and bFGF after 48h and decreased by TGFß1 and TGFß2 after 48 - 72h. All growth factors elevated significantly c-fos mRNA amount after 1h as c-myc mRNA after 24h. Effects on the mRNA expression of cell cycle proteins were observed significantly by bFGF after 24h stimulation. PDGF and bFGF upregulated the Ki67 mRNA expression and downregulated the mRNA of coll III and coll IV. TGFß1 and TGFß2 decreased the Ki67 mRNA expression and increased the coll III and coll IV mRNA amount. Conclusions: These results show that some of the examined cytokines induce contrary effects in respect of proliferation and extracellular matrix formation by hRPE cells in vitro. This knowledge may be used for therapeutical approaches.
This PDF is available to Subscribers Only