May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Oleyl-Phosphocholine Inhibits Proliferation of Human RPE Cells
K.H. Eibl; C.L. Schoenfeld; C.A. May; A.S. Neubauer; S. Priglinger; A. Kampik; U. Welge-Lussen
Author Affiliations & Notes
  • K.H. Eibl
    Ophthalmology, LMU Munich, Munich, Germany
  • C.L. Schoenfeld
    Ophthalmology, LMU Munich, Munich, Germany
  • C.A. May
    Anatomy, Friedrich Alexander University, Erlangen, Germany
  • A.S. Neubauer
    Anatomy, Friedrich Alexander University, Erlangen, Germany
  • S. Priglinger
    Anatomy, Friedrich Alexander University, Erlangen, Germany
  • A. Kampik
    Anatomy, Friedrich Alexander University, Erlangen, Germany
  • U. Welge-Lussen
    Anatomy, Friedrich Alexander University, Erlangen, Germany
  • Footnotes
    Commercial Relationships  K.H. Eibl, None; C.L. Schoenfeld, None; C.A. May, None; A.S. Neubauer, None; S. Priglinger, None; A. Kampik, None; U. Welge-Lussen, None.
  • Footnotes
    Support  German Research Fund DFG WE 2577/2-1
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 2957. doi:
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      K.H. Eibl, C.L. Schoenfeld, C.A. May, A.S. Neubauer, S. Priglinger, A. Kampik, U. Welge-Lussen; Oleyl-Phosphocholine Inhibits Proliferation of Human RPE Cells . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2957.

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Abstract

Abstract: : Purpose: To determine the effect of Oleyl-Phosphocholine (C18:1-PC) on human retinal pigment epithelium (RPE) proliferation and RPE mediated collagen matrix contraction in vitro. Methods: Cultured RPE cells of five human donors were treated with C18:1-PC in the presence of fetal calf serum. Proliferation was assessed by the tetrazolium dye-reduction assay (MTT). The effect of C18:1-PC on RPE mediated three-dimensional collagen gel contraction was determined. Cell viability was tested by the trypan blue exclusion assay. Results: C18:1-PC inhibited RPE proliferation and RPE mediated collagen matrix contraction in vitro. The antiproliferative and anticontractile effect of C18:1-PC increased in a dose-dependent manner. The IC50 concentrations of C18:1-PC was 26.5 µM ±1.3. Trypan blue staining revealed a toxicity below 5 % within the concentration interval tested. Conclusions: C18:1-PC inhibits proliferation of RPE cells and RPE-mediated matrix contraction in vitro at non-toxic concentrations. This could be a new strategy for prevention of RPE-mediated proliferative processes such as PVR.

Keywords: proliferative vitreoretinopathy • retinal pigment epithelium • pharmacology 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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