Purchase this article with an account.
H. Kou, P. Wu, C. Kuo, S. Liu, C. Wang; Inhibitory Effect of Roscovitine on the Proliferation of Rabbit Retinal Pigment Epithelium . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2959.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: It has been postulated that Roscovitine, a cyclin dependent kinase inhibitor, might inhibit the cellular proliferation by the cell cycle arrest. This in vitro study investigated the inhibitory effect of Roscovitine on the rabbit retinal pigment epithelium( RPE ) cell. Methods:Experiments using rabbit RPE cells from young pigmented rabbits were carried out using Roscovitine solved in DMSO ranging from 1 to 100 µM. Dulbecco's Modified Eagle Medium with 10% fetal calf serum (FCS) was used. Cell proliferation was measured by MTT assay and 5-bromo-2'-deoxy-uridine (BrdU) enzyme-linked immunosorbent assay. Results: Exposure of RPE to Roscovitine resulted in a concentration-dependent inhibition of cell proliferation. MTT assay showed that Roscovitine significantly inhibits cell proliferation in concentrations of 60 µM or more after 48- and 96-hour treatment in comparison with the untreated cells. It also shows that Roscovitine significantly inhibits cell proliferation in concentrations of 50 µM or more in the BrdU assay. Conclusions: This study indicates that inhibitory effect of Roscovitine on the RPE proliferation. Further studies will be required to determine the applicability of Roscovitine in the prevention of proliferative vitreoretinopathy.
This PDF is available to Subscribers Only