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H. Kwak, S.Y. Yu, W.H. Nam, J.S. Kim; The Expression of mRNA in the Vitreous of Proliferative Diabetic Retinopathy and Proliferative Vitreoretinopathy . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2961.
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Purpose: To find out whether vitreous takes part in producing cytokines and to detect the expression of mRNA of c-fos, PDGF(AA, BB), and HGF in the vitreous of proliferative diabetic retinopathy(PDR) and proliferative vitreoretinopathy(PVR). Methods: Undiluted vitreous samples were obtained during vitrectomy from 37 eyes of PDR patients and from 22 eyes of non-PDR patients. cDNA was synthesized from the vitreous and amplified using reverse transcriptase-polymerase chain reaction. The quantitation of mRNA was analyzed with densitometer. Results: ß-actin was expressed in 27 of 59 (37 PDR patients, 22 non-PDR patients) vitreous samples. The mRNA of c-fos and PDGF(AA, BB) was highly expressed in the 15 vitreous from PDR patients and 12 vitreous from non-PDR patients. The mRNA of HGF was slightly expressed in 6 vitreous samples. Conclusions: Our results suggest that the vitreous was involved in the production of cytokines related to the pathogenesis of PDR and PVR, and we need further study on the relations between the degree of the expression of mRNA and the amount of cytokines according to the disease stage.
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