May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Tissue Transglutaminase Cross-Linking of Laminin and Fibronectin in Proliferative Vitreoretinopathy (PVR) Membranes and Cultured Human Retinal Pigment Epithelium (RPE) Cells
U.C. Welge-Lussen; A. Neubauer; S.G. Priglinger; A. Fuchs; A. May; K. Eibl; C. Alge; A.S. Neubauer; A. Kampik
Author Affiliations & Notes
  • U.C. Welge-Lussen
    Dept. of Ophthalmology, Ludwig-Maximilians-University, Muenchen, Germany
  • A. Neubauer
    Dept. of Ophthalmology, Ludwig-Maximilians-University, Muenchen, Germany
  • S.G. Priglinger
    Dept. of Ophthalmology, Ludwig-Maximilians-University, Muenchen, Germany
  • A. Fuchs
    Dept. of Ophthalmology, Ludwig-Maximilians-University, Muenchen, Germany
  • A. May
    Dept. of Anatomy, Friedrich Alexander Universität Erlangen, Erlangen, Germany
  • K. Eibl
    Dept. of Anatomy, Friedrich Alexander Universität Erlangen, Erlangen, Germany
  • C. Alge
    Dept. of Anatomy, Friedrich Alexander Universität Erlangen, Erlangen, Germany
  • A.S. Neubauer
    Dept. of Anatomy, Friedrich Alexander Universität Erlangen, Erlangen, Germany
  • A. Kampik
    Dept. of Anatomy, Friedrich Alexander Universität Erlangen, Erlangen, Germany
  • Footnotes
    Commercial Relationships  U.C. Welge-Lussen, None; A. Neubauer, None; S.G. Priglinger, None; A. Fuchs, None; A. May, None; K. Eibl, None; C. Alge, None; A.S. Neubauer, None; A. Kampik, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 2962. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Tissue Transglutaminase Cross-Linking of Laminin and Fibronectin in Proliferative Vitreoretinopathy (PVR) Membranes and Cultured Human Retinal Pigment Epithelium (RPE) Cells
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      U.C. Welge-Lussen, A. Neubauer, S.G. Priglinger, A. Fuchs, A. May, K. Eibl, C. Alge, A.S. Neubauer, A. Kampik; Tissue Transglutaminase Cross-Linking of Laminin and Fibronectin in Proliferative Vitreoretinopathy (PVR) Membranes and Cultured Human Retinal Pigment Epithelium (RPE) Cells . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2962.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose: : In proliferative vitreoretinopathy (PVR) epi- and subretinal fibrocellular membranes containing modified retinal pigment epithelial (RPE) cells among others develop. In a previous study we have shown that RPE cells synthesize increased amounts of tissue transglutaminase (tTG) following treatment with transforming growth factor beta2 (TGF-ß2). tTG is an enzyme catalyzing irreversible crosslinking of extracellular matrix (ECM) components like fibronectin and laminin, both major components of PVR membranes. This study investigates the role of tTG, laminin and fibronectin for the accumulation of ECM by transglutaminases in these membranes. Methods: In vitro, RPE cells were treated with TGF-ß2, basic fibroblast growth factor, interleukin-6, and interleukin-1beta. Their effect on laminin and fibronectin expression was studied on Northern- and Western-blot analysis. Furthermore, a Western-blots for e-(g-glutamyl)-lysine were performed. In PVR-membranes localization of laminin, tTG, and the reaction product e-(g-glutamyl)-lysine was investigated immunohistochemically. Co-localization was studied with a fluorescence laser scanning microscope. Results: In RPE cells, laminin and fibronectin mRNA and protein formation could be stimulated by TGF-ß2 treatment. Additionally the reaction product of tTgase e-(g-glutamyl)-lysine was significantly increased by TGF-ß2. The other cytokines showed only little effect. In PVR-membranes, staining for laminin and fibronectin was co-localized with tTG and e-(g-glutamyl)-lysine. Conclusions: These findings underline the importance of tTG for ECM crosslinking in PVR-membranes. In vitro, the process can be stimulated by TGF-ß2, a growth factor known to be increased in the vitreous of PVR. Inhibition of tTG may offer a novel approach to preventing PVR development.

Keywords: retinal pigment epithelium • proliferative vitreoretinopathy • molecular biology 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept