Histopathology of the Optic Nerve Three Months Following Lamina Puncture

Abstract

Abstract: : Purpose: To study eye histopathology three months following puncture of the optic nerve adjacent to the central retinal vein. Methods: Puncture of the optic nerve was performed on one eye in three live rabbits using a pars plana approach. A modified microvitreoretinal blade with tip configurations 40 to 200 microns in width was inserted adjacent to the central retinal vein to a depth of 400 to 600 microns past the optic nerve face. The rabbits were sacrificed three months after the procedure and histopathology of the operated eyes was performed. Results: Histopathology of the eyes demonstrated that the created perivascular space in the optic nerve remained patent and significant in size three months following the procedure. Minimal fibrosis in the area surrounding the incision site was noted. There was no disruption of the vessel wall and no evidence of vitreous or papillary hemorrhage. Conclusions: The perivascular space that is created in the optic nerve following lamina puncture remains patent after three months. This may allow long-term decompression and reperfusion of the central retinal vein in patients with CRVO following this procedure.

Keywords: vascular occlusion/vascular occlusive disease • optic disc