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S.E. Lee, S.I. Pachydaki, M.P. Weisberg, S.R. Tari, A.M. Schmidt, S. Chang, G.R. Barile; Induction of PVR in a Murine Model: Dispase-induced Disease Progresses in the Presence of a Retinal Tear . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3010.
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Purpose: To report fundus findings with the dispase model of proliferative vitreoretinopathy(PVR) in murine eyes and to determine the role of retinal tear formation in advancing disease in this model. Methods: One eye of 25 C57B6 mice received the following treatments: intravitreal saline injection (N=6); intravitreal saline injection + retinal tear formation (N=7); intravitreal 0.008U dispase injection (N=5); intravitreal 0.008U dispase injection + retinal tear formation (N=7). Eyes were examined at one week, two weeks, and five weeks following the procedure, and they were graded at 5 weeks using a modified Fastenberg classification (grade 1: intravitreal membranes; grade 2: focal traction; grade 3: localized retinal elevation; grade 4: retinal elevation > 1 quadrant). Fundus images were captured using a 3 chip camera adapted for microscopic use. Results: The average grade of PVR was 0.00 in the saline group, 0.50 in the saline + tear group, 0.60 in the dispase group, and 2.29 in the dispase + tear group. Intravitreal membranes had differentiating characteristics depending upon the presence of dispase and the presence of a retinal tear. Dispase injection appeared to facilitate cellular migration of intravitreal membranes. In the presence of dispase, retinal tear formation appeared to increase the tractional force of intravitreal membranes. Florid retinal elevation only occurred in those eyes receiving dispase and a retinal tear. Conclusions: Dispase facilitates the development of PVR intravitreal membranes in murine eyes, and these membranes appear to be more advanced when these eyes also receive a retinal tear at the time of dispase injection.
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