Purchase this article with an account.
N. Koh, S. Saika, T. Miyamoto, S. Tanaka, A. Ooshima, Y. Ohnishi; A Model of Proliferative Vitreoretinopathy Induced by Lens Extraction in Mice . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3011.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose:To develop a mouse model of proliferative vitreoretinopathy (PVR) by inducing retinal detachment.Methods:Adult C57Bl/6 mice (n=26) were generally and topically anesthetized. The crystalline lens was removed through the corneal incision by the method of intracapsular extraction. Vitreous body was also removed with scissors. The vitreous cavity was filled with 1.0% sodium hyaluronate and the corneal incision was sutured with 10-0 nylon strings. After intervals of healing of 3, 5, 7 and 14 days, the mice were sacrificed and the enucleated eye was processed for histology. Deparaffinized sections were immunostained for α-smooth muscle actin (αSMA), an established marker of epithelial-mesenchymal transition (EMT) in retinal pigment epithelial cells (RPEs), collagen I and lumican.Results:: Neural retina was found to be detached from pigment epithelium in all the eyes examined at each interval. At Day 3 faint immunoreactivity for αSMA was seen in RPEs, whereas at and after Day 5, RPEs in the relatively peripheral area of subretinal space were elongated and markedly positive for αSMA, collagen I and lumican in association with the reduction of cytoplasmic pigment granules.Conclusions:Removal of lens and vitreous well induces retinal detachment and then induce a EMT in RPEs to model proliferative vitreoretinopathy in mice. Mouse RPEs undergo EMT within 3 days post-retinal detachment and express components to fibrous extracellular matrix.
This PDF is available to Subscribers Only