May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Practical Aspects of Human Fibrin Glue in Vitreoretinal Surgery
C. Azzolini; E. Balì; E. Alessandrini; C.T. Paties; S. Ciaccia; M. Prati; C. Tanzi; R. Brancato
Author Affiliations & Notes
  • C. Azzolini
    Ophthalmology, Vita-Salute San Raffaele University, Milan, Italy
  • E. Balì
    Ophthalmology, Univ of HS Pierre ULB, St Pierre, Belgium
  • E. Alessandrini
    Bioengineering, Politecnico, Milan, Italy
  • C.T. Paties
    Pathology, Vita-Salute San Raffaele University, Milan, Italy
  • S. Ciaccia
    Pathology, Vita-Salute San Raffaele University, Milan, Italy
  • M. Prati
    Pathology, Vita-Salute San Raffaele University, Milan, Italy
  • C. Tanzi
    Pathology, Vita-Salute San Raffaele University, Milan, Italy
  • R. Brancato
    Pathology, Vita-Salute San Raffaele University, Milan, Italy
  • Footnotes
    Commercial Relationships  C. Azzolini, None; E. Balì, None; E. Alessandrini, None; C.T. Paties, None; S. Ciaccia, None; M. Prati, None; C. Tanzi, None; R. Brancato, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3032. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Practical Aspects of Human Fibrin Glue in Vitreoretinal Surgery
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      C. Azzolini, E. Balì, E. Alessandrini, C.T. Paties, S. Ciaccia, M. Prati, C. Tanzi, R. Brancato; Practical Aspects of Human Fibrin Glue in Vitreoretinal Surgery . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3032.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose: Fibrin glues are studied in ophthalmology since 25 years, and in vitreoretinal surgery since 10 years. There is still no current application for the glue in vitreoretinal surgery. The aim of our study is to evaluate practical aspects in the use of fibrin glue in modern vitreoretinal surgery. Methods: Two ports pars plana vitrectomy (PPV) was performed in 4 pigs’ eyes enucleated within 8 hours. After the removal of all vitreous a retina hole was performed in the peripheral retina. In three eyes human fibrin glue was applied on the hole, and retina was then stabilized with perflourocarbon liquid (PFCL). In one eye retina was stabilized with PFCL and then fibrin glue was applied on the hole. After 5 minutes PFCL was removed in all eyes. Serial 3-µm sections were cut at intervals of 50 µm and stained with hematoxylin and eosin. Results: The injection of fibrin glue before the PFCL injection was suitable; in these eyes the hole was closed after PFCL removal. The injection of fibrin glue after PFCL injection was unsuitable because of fibrin glue uncontrollable diffusion and consequent sudden formation of a non usefull knot; in this eye the hole remained open. In two eyes there was leakage of the fibrin glue due to gravity and excessive fibrin glue had to be removed with the vitrectome. Histology showed the presence of fibrin glue under and around the hole margins in all eyes with a closed hole, in one eye no fibrin glue was found. Histology also showed that the fibrin glue can adhere to RPE as well as retina. Conclusion: Our result, even if in a very limited experience, showed that fibrin glue may close the hole if correctly applied. Main difficulties are unwanted diffusion of the fibrin glue due to gravity, the sudden polymerisation of the human fibrin glue and the lack of non dedicated surgical instruments. Much more studies and experimentation are necessary to reach the goal of usefull application of fibrin glue in vitreoretinal surgery.

Keywords: vitreoretinal surgery • retinal adhesion • retina 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept