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S.R. Tari, S.I. Pachydaki, S.E. Lee, W.M. Schiff, S. Chang, A.M. Schmidt, G.R. Barile; S100/Calgranulin and RAGE Expression in PDR & PVR . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3039.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Previous work demonstrated elevated vitreous levels of the receptor for advanced glycation end products (RAGE) and its proinflammatory binding ligands S100/calgranulins in eyes with proliferative diabetic retinopathy (PDR) and proliferative vitreoretinopathy (PVR). This study sought to examine the expression of RAGE and S100/calgranulins in epiretinal membranes ERMs of patients with proliferative retinopathy. Methods: Immunohistochemical staining was performed on paraffin sections of epiretinal membranes obtained at surgery from 6 eyes with PDR and 9 eyes with PVR. Polyclonal antibodies for RAGE and S100 were used for antigen detection and developed by DAB and/or FITC . Results: RAGE and its ligands S100/calgranulins are expressed in 5/6 epiretinal membranes of PDR and 8/9 epiretinal membranes in PVR . Immunoreactive cells in consecutive sections appeared to demonstrate expression of both RAGE and S100. Conclusions:In addition to its presence in the vitreous cavity of eyes with PDR and PVR, S100/calgranulins ligands are expressed in the ERMs of these disorders. The presence of these RAGE-binding ligands suggests a role for this inflammatory axis in the pathogenesis of these proliferative retinal disorders.
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