May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
S100/Calgranulin and RAGE Expression in PDR & PVR
S.R. Tari; S.I. Pachydaki; S.E. Lee; W.M. Schiff; S. Chang; A.M. Schmidt; G.R. Barile
Author Affiliations & Notes
  • S.R. Tari
    Ophthalmology, Columbia University, New York, NY, United States
  • S.I. Pachydaki
    Ophthalmology, Columbia University, New York, NY, United States
  • S.E. Lee
    Ophthalmology, Columbia University, New York, NY, United States
  • W.M. Schiff
    Ophthalmology, Columbia University, New York, NY, United States
  • S. Chang
    Ophthalmology, Columbia University, New York, NY, United States
  • A.M. Schmidt
    Surgery, Columbia University, New York, NY, United States
  • G.R. Barile
    Surgery, Columbia University, New York, NY, United States
  • Footnotes
    Commercial Relationships  S.R. Tari, None; S.I. Pachydaki, None; S.E. Lee, None; W.M. Schiff, None; S. Chang, None; A.M. Schmidt, None; G.R. Barile, None.
  • Footnotes
    Support  support: Research to Prevent Blindness
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3039. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      S100/Calgranulin and RAGE Expression in PDR & PVR
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      S.R. Tari, S.I. Pachydaki, S.E. Lee, W.M. Schiff, S. Chang, A.M. Schmidt, G.R. Barile; S100/Calgranulin and RAGE Expression in PDR & PVR . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3039.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose: Previous work demonstrated elevated vitreous levels of the receptor for advanced glycation end products (RAGE) and its proinflammatory binding ligands S100/calgranulins in eyes with proliferative diabetic retinopathy (PDR) and proliferative vitreoretinopathy (PVR). This study sought to examine the expression of RAGE and S100/calgranulins in epiretinal membranes ERMs of patients with proliferative retinopathy. Methods: Immunohistochemical staining was performed on paraffin sections of epiretinal membranes obtained at surgery from 6 eyes with PDR and 9 eyes with PVR. Polyclonal antibodies for RAGE and S100 were used for antigen detection and developed by DAB and/or FITC . Results: RAGE and its ligands S100/calgranulins are expressed in 5/6 epiretinal membranes of PDR and 8/9 epiretinal membranes in PVR . Immunoreactive cells in consecutive sections appeared to demonstrate expression of both RAGE and S100. Conclusions:In addition to its presence in the vitreous cavity of eyes with PDR and PVR, S100/calgranulins ligands are expressed in the ERMs of these disorders. The presence of these RAGE-binding ligands suggests a role for this inflammatory axis in the pathogenesis of these proliferative retinal disorders.

Keywords: diabetic retinopathy • immunohistochemistry • retinal degenerations: cell biology 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept